Isolation and Molecular Methods for the Identification of Fusarium solani from Solid Waste
Journal Title: STAR Journal - Year 2013, Vol 2, Issue 1
Abstract
A major goal in microbial ecology is to link speciï¬c microbial populations to environmental processes. Solid waste degradation is an imperative aspect of environmental processes mediated by microorganisms individually or symbiotically. In the current study an attempt has been made to isolate and identify a microorganism which can degrade the hazardous xenobiotic compound propaxur commonly known as baygon from solid waste by both in vitro and in silico approaches. 28s rDNA gene has indicated the organism as Fusarium solani. Further studies were extended through bioinformatics approaches like BLAST (Basic Local Alignment Search Tool) and MSA (Multiple Sequence Analysis) to determine the relative phylogeny of this organism. This approach has shed light on evolutionary relationship among the organisms taken in the study. By this approach we have identiï¬ed the Fusarium solani.
Authors and Affiliations
Chetan DM| Department of Biotechnology NMAM Institute of Technology- Nitte, Karnataka, India, Nataraja S| Department of Botany Sahyadri Science College, Kuvempu University-Shimoga, Karnataka, India, Krishnappa M| Department of studies and research in Applied Botany, Kuvempu University, Shankaraghatta, Karnataka, India, Bharath BR| Department of Biotechnology and Bioinformatics, Jnanasahyadri, Kuvempu University, Shankaraghatta, Karnataka, India, Ravikumar Patil HS| Department of Biotechnology NMAM Institute of Technology- Nitte, Karnataka, India, Raghavendra HL| College of Health and Medical Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia
Keywords
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- DOI http://dx.doi.org/10.4314%2Fstar.v2i1.98843
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