Isolation and Screening of Amylase from Moulds Associated with the Spoilage of Some Fermented Cereal Foods
Journal Title: Microbiology Research Journal International - Year 2015, Vol 5, Issue 4
Abstract
Aims: The aim of the study is to isolate moulds from spoilt samples of three fermented cereal foods (ogi, eko, pap) and screen the mould for amylase production. Study Design: Primary screening for amylase production by the mould isolates was determined on starch agar. Isolates having the widest zones of hydrolysis were further screened by Solid state fermentation [SSF] Methodology: Molds were isolated on Sabouraud Dextrose Agar [SDA] incubated at 28°C±2°C for 72h and identified by standard microbiological methods. Screening of moulds for amylase activity was determined on starch agar medium and flooed with gram’s iodine. The isolates were screened quantitatively for the production of α-amylase and glucoamylase using Solid state fermentation [SSF] while the enzyme activities were determined spectrophotometrically. Results: Mean mould population observed on the spoilt food samples ranged from 5.06 log cfu/g for ogi to 5.80 log cfu/g for eko wrapped in leaves. Twenty seven moulds isolated from the samples were identified as Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Penicillium citrinum and Rhizopus stolonifer. Majority (70.4%) of the isolated mould produced amylase on starch agar indicated by clear zones around the colonies. Diameter of the zone of hydrolysis ranged from 3.0 mm for Aspergillus niger EL4 to 22.0 mm for Aspergillus flavus OG1. The highest amylase activity was 30.8% from A. flavus OGI while the least was 21.2% from A. flavus EN4. Glucoamylase activities observed were between 100 U/ml and 240 U/ml. respectively. Conclusion: This study contributes to catalogued local fungal isolates that can be used for amylase production and provides additional information to support future research about the industrial enzymes potential of these microorganisms. More work is however needed to determine the optimal production conditions of the enzymes.
Authors and Affiliations
A. M. Omemu, G. Bamigbade, A. O. Obadina, T. M. Obuotor
Keywords
Related Articles
- EP ID EP354453
- DOI 10.9734/BMRJ/2015/11190
- Views 77
- Downloads 0
Diversity of Plasmid Profile in Multi-drug Resistant Non- E. coli Intestinal Flora Lacking Association with Resistance Phenomenon
Aims: To study the plasmid diversity of intestinal non- Escherichia coli community, describe their potential as a reservoir of antibiotic resistance phenomenon and to find any association of resistance phenomenon with th...
Poultry Environment as a Reservoir of Antimicrobial Resistant Bacteria – A Nigerian Story
Aims: This study set out to help define the role of the poultry environment as a reservoir of drug resistant bacteria in Nigeria. Introduction: The poultry environment has been acclaimed as a potential source of antimicr...
Activity Guided Fractionation with Antimicrobial Evaluation of Pergularia tomentosa L. (Asclepiadacea) Whole Plant
This research work aimed to present the activity guided fractionation with antimicrobial evaluation from both crude extract and various fractions obtained from Pergularia tomentosa L. whole plant. The P. tomentosa L. who...
Antifungal Resistance Patterns, Virulence Attributes and Spectrum of Oral Candida Species in Patients with Periodontal Disease
Aims: To observe the antifungal resistance pattern, virulence attributes and spectrum of Candida species in oral cavities of patients with periodontal diseases and healthy individuals. Study Design: A total number of 52...
Poly-β-hydroxybutyrate: A Biodegradable Polyester, Biosynthesis and Biodegradation
Bioplastic can be degraded in nature with time and produce carbon dioxide as an end product. It reduces the environmental pollution created by synthetic plastic. Biodegradable plastic can be used to make plastic bags and...