Isolation and Screening of Cellulolytic Bacteria Inhabiting Different Environment and Optimization of Cellulase Production
Journal Title: Universal Journal of Environmental Research and Technology - Year 2013, Vol 3, Issue 1
Abstract
The cellulase producing bacteria were isolated from various region including paper industry waste, municipal waste, sugarcane farm, garden, and wood furnishing region. Total 34 isolates were obtained by the primary screening technique from which 11 isolates were showing maximum cellulase activity. Potential isolates were obtained from wood furnishing region and paper industry waste. These 11 isolates were then evaluated by secondary screening for enzyme production. Among these 11 isolates CDB27 and CDB30 were selected as most efficient enzyme producers and their specific enzyme activity in the crude sample was found to be 6.0U/mg and 8.4 U/mg and of partially purified sample was found to be 6.97 U/mg and 9.3 U/mg respectively. Isolates were tentatively characterized on the basis of their cultural and morphological and biochemical characteristics, CDB27 and CDB30 were identified to be Pseudomonas spand Bacillus sp respectively. Further partial purification of the cellulase enzyme was carried out by ammonium sulfate precipitation followed by dialysis. Optimization of different parameters was carried out for the production of cellulase by both efficient isolates. The maximum enzyme producing isolate CDB30 was used to check biodegradation properties at laboratory scale.
Authors and Affiliations
Shaikh N. M. ,Patel A. A. , Mehta S. A. 2, Patel N. D.
Keywords
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