ISOLATION AND SCREENING OF INDUSTRIALLY IMPORTANT POLYGALCTURONASE PRODUCING FUNGI FROM THE MANGROVE SOILS OF KRISHNA DISTRICT ANDHRA PRADESH
Journal Title: International Journal of Agriculture Sciences - Year 2017, Vol 9, Issue 19
Abstract
For the present study, soil samples were collected from mangrove areas of Gilakaladindi and Malakayalanka of Krishna District. Soil samples were serially diluted and plated on pectin agar plates for isolation of Fungi. The isolates were identified as Penicillium citrinum RR 101(KU 613360) and Aspergillus oryzae RR 103(KU 613361) Penicillium griseofulvum RR104 (KU613362) and Aspergillus sp RR102. Qualitative assay of exo-polygalacturonase of fungal isolates was carried out by growing them on to the pectin agar plates and the zone of hydrolysis around the fungal colonies was measured after flooded with iodine solution. Aspergillus sp. RR 102 showed the maximum zone of hydrolysis (14 mm) after 192 h at 370C. Maximum Polygalacturonase enzyme activity was recorded in Aspergillus sp RR 102 (2.40 IU/ml).
Authors and Affiliations
K. RAVI, M. RAGHU RAM
Keywords
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- EP ID EP172026
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