Isolation and structural elucidation of antifungal compounds from Curcuma amada
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2019, Vol 12, Issue 3
Abstract
Objective: To isolate and identify the antifungal compounds from Curcuma amada. Methods: The antifungal activity was measured by the diameter of colonies grown on Petri dish, microscopic observation, and CLSI microdilution methods. The antifungal compounds were isolated through bioactivity guided purification by using silica gel and high-performance liquid chromatography. Structural identification of the antifungal compounds was conducted using 1H NMR, 13C NMR, and liquid chromatography-tandem mass spectrometry. Results: The purified antifungal compounds were zederone and furanodienone. These two compounds showed dose-dependent antifungal activity against Fusarium solani sensu lato. The concentration required for 50% growth inhibition (IC50) of FSSL ranged from 115 to 129 μM and 82 to 91 μM for zederone and furanodienone, respectively. Conclusion: This study suggested that the isolated compounds from Curcuma amada could be promising natural antifungal agents to control the diseases caused by Fusarium solani sensu lato.
Authors and Affiliations
Md. Amzad Hossain
Keywords
Related Articles
- EP ID EP489955
- DOI 10.4103/1995-7645.254938
- Views 75
- Downloads 0
Prevalence of hydatidosis in livestocks in Chakwal District of Pakistan
Background: Hydatidosis is a zoonotic parasitic disease and a serious human and animal health problem in many endemic areas of the world. It is caused by the tapeworm parasite of Echinococcus granulosus sensu lato. This...
Plants used in traditional medicine for treatment of malaria by Tetun ethnic people in West Timor Indonesia
Objective: To document the medicinal plants used for the treatment of malaria by the Tetun ethnic people in West Timor–Indonesia. Methods: The ethnobotany and anthropology methods were used in the field surveys. Ninety f...
In vitro inhibitory analysis of consensus siRNAs against NS3 gene of hepatitis C virus 1a genotype
Objective: To explore inhibitory effects of genome-specific, chemically synthesized siRNAs (small interference RNA) against NS3 gene of hepatitis C virus (HCV) 1a genotype in stable Huh-7 (human hepatoma) cells as well a...
Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus
Objective: To evaluate the total phenolic content and compare the antioxidant activity of various solvent extracts and fractions from the aerial parts of Coronopus didymus through various assays. Methods: Total phenolic...
A hiddenly high hepatitis C virus related liver disease burden among Chinese patients with non-liver disease complaints: A hospital based study from 2013 to 2017
Objective: To determine the frequency of anti-HCV antibody positivity in patients with nonliver disease complaints, to explore whether anti-HCV positive patients had been properly advised and visited hepatologists for fu...