Isolation, Characterization And Antibacterial Activity Of Bacteriocin Producing Bacteria From Dairy Effluents
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 4
Abstract
The intent of study is to determine the antimicrobial activity of bacteria producing bacteriocin isolated from dairy effluents .Several bacterial isolates were picked from the samples by growing on selective media(MRS Agar media).Bacteriocin producing organisms were screened by agar spot assay test.Six isolates are able to produce bacteriocin whose antibacterial activity was analysed by Agar well diffusion assay test against indicator organisms which are pathogenic.Bacillus cereusATCC1178,Staphylococcus aureus MTCC87 were more sensitive to the isolates than Salmonella typhi MTCC734 and Pseudomonas aeruginosa MTCC424. The antibacterial protein bacteriocin was characterized based on different pHvalues(lactic acid production), catalase test. By biochemical and molecular characterization the best bacteriocin producing strain was identified as Paenibacillus lactis. Analysis of the 16S rRNA gene of the MSB2 type placed these isolate within the genus Paenibacillus, Moreover, over 99% similarity was observed to the 16S rDNA sequence of MB 1871 a strain isolated previously from raw and heat treated milk. This study reveals the possibility of isolating Bacteriocin strains from dairy effluents and using them to control pathogenic bacteria.
Authors and Affiliations
Y Evangelin, K Kasturi
Keywords
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