Isolation, Characterization And Antibacterial Activity Of Bacteriocin Producing Bacteria From Dairy Effluents
Journal Title: IOSR Journal of Pharmacy (IOSRPHR) - Year 2018, Vol 8, Issue 4
Abstract
The intent of study is to determine the antimicrobial activity of bacteria producing bacteriocin isolated from dairy effluents .Several bacterial isolates were picked from the samples by growing on selective media(MRS Agar media).Bacteriocin producing organisms were screened by agar spot assay test.Six isolates are able to produce bacteriocin whose antibacterial activity was analysed by Agar well diffusion assay test against indicator organisms which are pathogenic.Bacillus cereusATCC1178,Staphylococcus aureus MTCC87 were more sensitive to the isolates than Salmonella typhi MTCC734 and Pseudomonas aeruginosa MTCC424. The antibacterial protein bacteriocin was characterized based on different pHvalues(lactic acid production), catalase test. By biochemical and molecular characterization the best bacteriocin producing strain was identified as Paenibacillus lactis. Analysis of the 16S rRNA gene of the MSB2 type placed these isolate within the genus Paenibacillus, Moreover, over 99% similarity was observed to the 16S rDNA sequence of MB 1871 a strain isolated previously from raw and heat treated milk. This study reveals the possibility of isolating Bacteriocin strains from dairy effluents and using them to control pathogenic bacteria.
Authors and Affiliations
Y Evangelin, K Kasturi
Keywords
Related Articles
- EP ID EP381206
- DOI -
- Views 73
- Downloads 0
Development and Validation of Stability Indicating Method for Simultaneous Estimation of Cefepime and Tazobactam Injection using RP-UPLC Method
This research manuscript describes simple, sensitive, accurate, precise and repeatable RPUPLC method for the simultaneous determination of Cefepime (CEFE) and Tazobactam (TAZ) Injection in combine dosage form. T...
Varied Virological Response of Patients with Chronic Hepatitis C against the Treatment of Pegylated Interferon- α and Ribavirin
[b]INTRODUCTION : [/b]Hepatitis C Virus (HCV) is the leading cause of death throughout the world. The standard of care for the treatment of chronic hepatitis C is combination therapy with Pegylated Interferon (PEG-IFNα 2...
Correlation of Estrogen and Progesterone Receptor expression in Breast Cancer
Breast cancer is the most common malignant tumor among women. It accounts for 22% of all female cancers; more than twice the prevalence of cancer in women at any other site. Immunohistochemistry (IHC) is now the gl...
Development and Validation of a Simple UV Spectrophotometric Method for the Determination of Cefotaxime Sodium in Bulk And Pharmaceutical Formulation
The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefotaxime sodium in bulk and pharm...
An Audit of the Management and Associated Contextual Correlates of Clinical Presentations of Breast Cancer in a Tertiary Hospital in South West Nigeria
ABSTRACT : Breast cancer is a worldwide disease resulting in many deaths. This study investigated the management of breast cancer with respect to treatment modalities and other clinical factors in the study&nb...