Isolation, Identification and Antimicrobial Susceptibility Pattern of Escherichia Coli Isolated From Urine Samples at Tertiary Care Hospital
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 10
Abstract
Urinary tract infection (UTI) is the most common bacterial infection caused by both gram negative bacilli and gram positive cocci among which E. coli remains the leading uropathogens involving both men and women of all age groups. To isolate, identify and study the Antimicrobial Susceptibility Pattern of E. coli isolated from urinary tract infected patients of both sex of all age group. A sterile urine samples were collected from various clinical departments with suspected urinary tract infection and transported to central laboratory. The samples were subjected to direct microscopy, culture and biochemical tests for isolation of bacterial pathogens. Organisms were identified and Antimicrobial susceptibility testing was performed on Muller-Hinton agar by Kirby-Bauer disk diffusion method following CLSI (Central Laboratory Standard Institute) guidelines. A battery of antibiotics includes Gentamycin (10µg), Tobramycin (10µg), Ciprofloxacin (5µg), Ceftazidime (30µg), Piperacillin-Tazobactam (100/10µg), Ceftriaxone (30 µg), cefuroxime (30µg), Imipenem (10µg), Trimethoprim-sulfamethoxazole (1.25/23.75 µg), Ampicillin (10µg), Amoxicillin-clavulanic acid (20/10µg) and Nitrofurantoin (300 µg). A total of 486 urine samples were processed in central laboratory out of which 88 were isolated and identified as E. coli in both sex of all age groups. The E. coli isolates, were highly susceptible to Imipenem (95%), Piperacillin-Tazobactam (90%), Tobramycin (80%), Gentamicin (75%), nitrofurantoin 94%, with maximum resistance to Ampicillin (75%) and Amoxicillin-Clavulanic acid (68%). In this study the antimicrobial susceptibility to uropathogenic E.coli were studied and most classes of antibiotics were found to be sensitive.
Authors and Affiliations
Dr. Sathyavathy K, Dr. Kiran Madhusudhan B, Dr. Chitralekha Saikumar
Keywords
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