Isolation, Identification and Optimization of Alkaline Amylase Production from Bacillus cereus Using Agro-Industrial Wastes
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 1
Abstract
Amylase with maximum activity at alkaline pH prefers most industrial applications. The present study investigate amylase production and optimization by bacterial strain isolated and identified from rice mill waste dumped soil. The potent isolate KR9 was identified based on biochemical characteristics and 16S rRNA gene analysis confirmed that this isolate belonged to the genus Bacillus and it was most closely related to B. cereus (99 % similarity). The selected isolate B. cereus KR9 was optimized for pH, growth period and carbon and nitrogen sources. The highest activity of amylase was obtained at alkaline pH 10.0 after 48 hours (225.2±0.63 U/ml) incubation. Optimization experiment performed with synthetic medium showed starch and peptone as suitable carbon and nitrogen source for amylases production (221±0.77 U/ml). B. cereus KR9 was further evaluated for amylase production using Solid State Fermentation and Submerged Fermentation with wheat bran, rice straw, paddy straw and sugarcane bagasse. The maximum amylase production of 281.1±0.65 U/ml with specific activity of 1.11±0.01 U/mg was observed with wheat bran using Submerged State Fermentation. This work has impending utilizing of agro-industrial waste affords cost effective and ecofriendly method for amylase production.
Authors and Affiliations
M. Krishma, S. Radhathirumalaiarasu
Keywords
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- EP ID EP566288
- DOI 10.20546/ijcmas.2017.601.003
- Views 65
- Downloads 0
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