Isolation, Molecular Identification and Antibiotic Resistance of Enterococcus faecalis from Diseased Tilapia
Journal Title: International Journal of Current Microbiology and Applied Sciences (IJCMAS) - Year 2017, Vol 6, Issue 6
Abstract
Aquaculture is one of the fastest growing food production sectors globally. Tilapia is the second widely farmed fish species in the global fish production. Enterococcus sp. is one of the leading causes of nosocomial infections in urinary tract, surgical wound and endocarditis in humans. These infections can be hard to treat because of the rising incidence of multiple antibiotic-resistances. The spread of antibiotic resistance has become a major concern in both human and veterinary medicine. In this study, we isolated and characterized an Enterococcus faecalis isolate from a diseased cultured Tilapia. Initial isolation of putative E. faecalis was carried out on streptococcus enrichment broth for 36 h. Characteristic, gram-positive, black color colony was selectively sub-cultured and subsequently identified by 16 rRNA sequencing analysis as Enterococcus faecalis (Genbank Acc no. KT877352). Furthermore, when the isolate was subjected to profiling against 16 antibiotics, it was found to be highly resistant to amoxyclave, ampicillin, erythromycin, gentamicin, kanamycin, nitrofurantoin, penicillin G, streptomycin, sulphafurazole, and vancomycin. The findings of present study showed that E. faecalis infects the cultured Tilapia species and the isolate (SRLFDA/TIL-1/15) possess multiple antibiotic resistance, which emphasizes the urgent need for targeted alternate bio-control strategies for control of emerging diseases like Enterococcus sp. infection in Tilapia culture.
Authors and Affiliations
Uma Arumugam, Nattan Stalin, Gnanadesika Pandian Rebecca
Keywords
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- EP ID EP570936
- DOI 10.20546/ijcmas.2017.606.016
- Views 54
- Downloads 0
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