ISOLATION OF FOUR FUROQUINOLINE ALKALOIDS FROM TECLEA NOBILIS AND THEIR ACTIVITY AGAINST SCHISTOSOMA MANSONI MIRACIDIA
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 4
Abstract
Schistosomiasis is one of the neglected diseases affecting approximately 200 million people worldwide. In this research, we isolated and evaluated the antimiracidial activity of secondary metabolites from Teclea nobilis (Rutaceae) which has been used ethnomedicinally against helminthes. Ethyl acetate extract was fractionated over silica gel column chromatography yielding four furoquinoline alkaloids which were identified through analysis of their 1D and 2D NMR, mass spectrometry data and comparison with literature data as; tecleoxine A, methylnkolbisine B, kokusaginine C and nkolbisine D. Serial dilutions of these compounds were exposed to Schistosoma mansoni miracidia. Compounds A, B and C were tested for bioassay and analyzed as a mixture because they were not resolved individually into purified forms due to their minimal amounts and close Rf values. Miracidial mortality was determined after 30 minutes under a dissecting microscope. Log probit regression analysis at 95% confidence level was used to calculate LC50 and LC90 using IBM SPSS version 21.0 software. Compounds A, B and C mixture were the most potent with LC50 and LC90 values of 270.2 and 690.9 ppm, respectively. Synergism could have been the reason for the high potency. Compound B was the least potent with mortality recorded LC50 and LC90 values of 287.9 and 631.7 ppm, respectively. These findings indicate that these compounds could be useful as lead compounds for the development of schistosomicide. Also, considering that a large number of human populations, mostly from poor backgrounds, suffer chronic schistosomiasis, the discovery of plant compounds that may control schistosomiasis could be of great value.
Authors and Affiliations
Mark K. Njogu| Department of Chemistry, Egerton University, P.O. Box 536, Egerton 20115, Kenya, Josphat C. Matasyoh*| Department of Chemistry, Egerton University, P.O. Box 536, Egerton 20115, Kenya, Alfred C. Kibor| Department of Animal Sciences, Egerton University, P.O. Box 536, Egerton 20115, Kenya
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