Isolation of microorganisms and screening of heavy metals from Municipal Council’s treated sewage from Nakuru sewage treatment plant in Nakuru County, Kenya
Journal Title: Scientific Journal of Microbiology - Year 2014, Vol 3, Issue 12
Abstract
Diarrhoeal cases due to taking of contaminated water are on the increase Nakuru County. They are a leading cause of human mortality and morbidity. The current study was aimed at isolating microorganisms from Municipal’s council treated sewage and screening it for heavy metals followed by testing the efficacy of Moringa stenopetala in removing the heavy metals and treating the sewage against the microbial isolates. In addition, susceptibility test of the microbial isolates to antibiotics and Moringa stenopetala was also carried out. A total of 492 samples were collected out of which 46 % (226/492) were positive for all the microorganisms isolated and heavy metals. Standard methods were used in isolating microorganisms and screening of the sewage for heavy metals. Antimicrobial susceptibility test was carried out using Karby Bauer’s disk diffusion test. The percentage reduction of heavy metals was Cadmium (97 %), nickel (97 %), lead (83.17 %) and Chromium (88 %) after treatment of the Municipal council’s sewage with Moringa stenopetala. In addition, the microbial isolates reduced by 95.3 % after treatment with Moringa stenopetala. There was significant difference in both heavy metals (p=0.036) and microbial isolates (p=0.02) before and after treatment of Municipals council treated sewage with Moringa stenopetala. There was also a significant difference (p=0.01) in susceptibility patterns of the microorganisms against antibiotics and Moringa stenopetala. Treated municipal council’s sewage is highly contaminated with microorganisms that can cause diseases in addition to having highly harmful heavy metals if ingested in food. There is need for proper sewage treatment in Nakuru town in addition to in cooperating Moringa stenopetala in sewage treatment in the town.
Authors and Affiliations
P. N. Waithaka*| Department of Biological Sciences, St. Joseph’s Kirima School, P. O. Box 17991, Nakuru, Kenya., J. M. Maingi| Department of Microbiology, Kenyatta University, P. O. Box 43844, Nairobi, Kenya., A. K. Nyamache| Department of Microbiology, Kenyatta University, P. O. Box 43844, Nairobi, Kenya.
Keywords
Related Articles
- EP ID EP1197
- DOI 10.14196/sjm.v3i12.1814
- Views 488
- Downloads 24
Seroprevalence of measles IgM in children 5-12 years from selected primary schools in Giwa Local Government Area, Zaria, Kaduna state
Seroprevalence of Measles IgM was carried out among children age 5-12 years in Giwa, Kaduna State. The test was carried out using Enzyme Linked Immunosorbent Assay (ELISA) method. Of the 90 (100%) samples assayed for the...
Dynamic of IS6110 transposase, transframe protein responsible for IS6110 element transposition in Mycobacterium tuberculosis strains
Transposition of IS6110, the Mycobacterium tuberculosis Complex specific insertion sequence, is mediated by the complex open reading frame AB (orfAB) transframe protein or transposase. Transposase has the crucial role to...
Molecular detection of inv A and spv virulence genes in Salmonella typhimurium isolated from human and animals in Iran
In the present study, multiplex Polymerase Chain Reaction (m-PCR), simple PCR assay was used to confirm Salmonella typhimurium and detect Salmonella plasmid virulence (spvA, spvB, spvC) genes in human, bovine and poultry...
Comparison of capillary and venous blood using blood film microscopy in the detection of malaria parasites: A hospital based study
Malaria mainlydue to Plasmodium falciparum isassociated with great morbidity and mortality in Sub-Saharan Africa especiallyin children under 5 years. In order to eradicate the disease and avoidcomplications that may aris...
Recurrent appearance of foot and mouth disease virus (FMDV) in Saudi Arabia
There are seven immunologically distinct serotypes of FMDV (O, A, C, Asia 1, SAT 1, SAT 2 and SAT 3), most of them were detected and identified in different regions of Saudi Arabia. FMDV serotype O was the most frequent...