ISOLATION OF PHYTOCONSTITUENTS AND IN VITRO ANTILITHIATIC BY TITRIMETIC METHOD, ANTIOXIDANT ACTIVITY BY 1,1- DIPHENYL -2- PICRYL HYDRAZYL SCAVENGING ASSAY METHOD OF ALCOHOLIC ROOTS & RHIZOMES EXTRACT OF HEDYCHIUM CORONARIUM J. KOENIG PLANT SPECIES
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2015, Vol 8, Issue 4
Abstract
ABSTRACTObjective: The herbal medicines do not have any side effect and it is cost effective and safest medicine from an ancient periods. Urolithiasis or Lithiasisis a consequence of complex physical processes. The major factors are supersaturation of urine with the offending salt and crystallization. Crystalsretained in kidney can become nucleus for stone formation. This process is synonymously known as Urolithiasis or Lithiasis or Nephrolithiasis orKidney stones or renal calculi. Calcium-containing stones are the most common comprising about 75% of all urinary calculi, which may be in theform of pure calcium oxalate (50%) or calcium phosphate (5%) and a mixture of both (45%). In this study, ethanolic & aqueous extracts of roots andrhizomes of Hedychium coronarium J. Koenig plant were evaluated for their potential to dissolve experimentally prepared kidney stones like calciumoxalate by titrimetic method with an invitro model and Antioxidant activity performed by DPPH scavenging assay method. Phytoconstituents werealso isolated by chromatographic techniques of this plant species.Method: For performing invitro antilithiatic activity titrimetic method was adopted and for antioxidant activity 1,1-diphenyl-2-picryl hydrazyl(DPPH) scavenging assay method was adopted. Phytoconstituents were isolated by column and thin layer chromatographic techniques.Results & Conclusion: Ethanolic roots & rhizomes extract of this plant produced highest dissolution of stones when compare to standard drugcystone and at 10 mg. concentration. Also this study showed alcoholic extract of roots & rhizomes of Hedychium coronarium J. Koenig plant in higherconcentration possess best antioxidant potential as compare to standard ascorbic acid with IC50 value 9.0 and 18.9 μg/ml. for ascorbic acid andalcoholic extract respectively. Isolated phytoconstituent from alcoholic extract of this plant was 8a, hydroxy hedychilactone and its structure wasconfirmed by IR, NMR and Mass spectroscopic datas.Keywords: Hedychium coronarium J. Koenig, Urolithiasis, Kidney stones, Calcium oxalate, Antioxidants, 1,1- diphenyl-2-picryl hydrazyl etc.
Authors and Affiliations
Chandra Shekhar Tailor, Anju Goyal
Keywords
Related Articles
- EP ID EP605790
- DOI -
- Views 78
- Downloads 0
PHYTOCONSTITUENTS, PHARMACOLOGICAL ACTIVITY, AND MEDICINAL USE OF LEPIDIUM SATIVUM LINN.: A REVIEW
Herbal medicines are in nice demand within the developed additionally as developing countries for primary aid due to their wide biological and medicative activities, higher safety margins, and lesser prices. Lepidium sat...
IMMUNOHISTOCHEMICAL EXPRESSION OF THE PROMISING THERAPEUTIC TARGET (HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2/NEU) IN IRAQI PATIENTS WITH MEDULLOBLASTOMA
Objectives: With the advent of ongoing novel modalities toward the treatment of human epidermal growth factor receptor 2 (HER2)/NEU - positive malignancies, the serious side effects of chemoradiotherapy have been minimiz...
DOUBLE-BLIND RANDOMIZED CLINICAL TRIAL OF KAEMPFERIA GALANGA L EXTRACT AS AN ANTI-INFLAMMATION (PROSTAGLANDIN E2 AND TUMOR NECROSIS FACTOR ALPHA) ON OSTEOARTHRITIS
Objective: The purpose of this study was to compare the effectiveness of the rhizome extract against inflammatory markers such as tumor necrosis factor (TNF)-alpha and prostaglandin E2 (PGE2) with meloxicam in patients w...
BIOCHEMICAL SCREENING OF MARINE MICROMONOSPORA MARINA KPMS1 (MH036351) AND ITS ANTIBACTERIAL ACTIVITY AGAINST MULTIDRUG RESISTANT BACTERIA
Objective: The present investigation aimed at the screening of pharmaceutically potential antimicrobial metabolite isolated from marine Micromonospora sp. to combat the multidrug‑resistant bacteria. Materials and Method...
PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY ACTIVITY OF FRACTIONS FROM SAMBUNG RAMBAT (MICANIA CORDATA) LEAF
Objective: The present study is to evaluate the anti-inflammatory activity of various fractions from Sambung Rambat leaf (Micania cordata) using ethyl acetate, hexane, and water solvent as the solvent.Methods: Investigat...