ISOLATION OF PHYTOSTEROL FROM PALM FATTY ACID DISTILLATE (PFAD) USING SOXHLET MODIFICATION
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2018, Vol 5, Issue 4
Abstract
Objective: To isolate phytosterol compounds from PFAD to obtain pure isolates. Methods: Saponification was used to separate the sterilized part (polar extract) containing ALB and TAG with unsabbed portions (non-polar extract) containing phytosterol. This non-polar saponification extract was isolated using modified soxhlet with silica gel binder. The identification of isolate purity was conducted using Fourier Transform Infrared (FT-IR) and Gas Chromatography Mass Spectroscopy (GC-MS). Results: Saponification results obtained non-polar crude extract (unsabbed portion) with a concentration of 2,683 ppm. The result identification of phytosterol isolate with FT-IR showed the similarity of functional group between phytosterol standard and isolate. However, peak appeared in the isolate at the wave number 1741 cm-1 showing the carbonyl group C=O. Meanwhile, identification using GC-MS showed isolate purity rate was 80% to the standard. The phytosterol content contained in the isolate was 4,035 ppm. Conclusion: The results of this study indicated that the obtained phytosterol isolate had a fairly good purity with a rate of 80%. The method used is also very beneficial because it can save the solvent, increase the amount of results obtained and short in time.
Authors and Affiliations
Ovalina Sylvia Br. Ginting
Keywords
Related Articles
- EP ID EP635712
- DOI -
- Views 140
- Downloads 0
ADVANCES IN MEDICAL MANAGEMENT OF PROSTATE CANCER: A REVIEW
Prostate cancer remains the number 1 male cancer in terms of incidence after skin cancer, and the number 2 cancer mortality; with a seemingly increasing trend in advanced and resistant prostate cancers. This report shows...
TRANSIENT ISCHEMIC ATTACK AS A PREDICTOR OF STROKE
Transient ischemic attack (TIA) is a brief episode of neurological dysfunction caused by focal brain or retinal ischemia, with clinical symptoms typically lasting less than one hour, and without evidence of acute infarct...
PERSISTENT RISE OF BHCG AFTER SURGICAL TREATMENT OF ECTOPIC PREGNANCY
Keycontent Bhcg measurements after treatment of ectopic pregnancy is justified to rule out persistent trophoblastic activity. Management of persistent rise of Hcg after surgical treatment of ectopic remains a clinica...
HEPATOPROTECTIVE EFFECT OF ROSEMARY EXTRACT AGAINST ASPARTAME-INDUCED LIVER TOXICITY IN MALE RATS
There is a growing interest in using natural antioxidants to treat various pathological tissue conditions considering the role of oxidative stress in their pathogenesis. Rosemary (Rosmarinus officinalis L., Lamiaceae) is...
HERBAL GEL CONTAINING CORM EXTRACT OF PISANG KEPOK (MUSA BALBISIANA) PROMOTE HAIR GROWTH OF RABBIT
Current technologies and therapies of hair disorders are not yet satisfying people suffer from hair loss, so that efforts in seeking effective medicine for promoting hair growth are still continues. This study intended t...