Isolation of Sabin-like Polioviruses from Sewage in Poland
Journal Title: Polish Journal of Microbiology - Year 2018, Vol 67, Issue 1
Abstract
As a complement to the active search for cases of acute flaccid paralysis, environmental sampling was conducted from January to December 2011, to test for any putative polio revertants and recombinants in sewage. A total of 165 environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture (L20B, RD and Caco-2) followed by neutralization and reverse-transcription polymerase chain reaction. Out of the 31 CPE positive samples, 26 contained one and 5 two different serotypes, yielding a total of 36 PVs. The microneutralization test revealed the presence of 7, 10 and 19 strains belonging to poliovirus serotype 1, 2 and 3, respectively. The genomic variability of 36 poliovirus strains was examined by the restriction fragment length polymorphism assay (RFLP). By combined analyses of two distant, polymorphic segments of the viral genome, one situated in the capsid protein VP1 coding region and the other in the 3D-polymerase coding region, we screened for the putative poliovirus revertants and recombinants. All detected PVs were classified as vaccine strains on the basis of RFLP-VP1 test. None of wild-type PVs or vaccine derived polioviruses were detected. RFLP assay also revealed the presence of 11 recombinants in 3D-polymerase coding region. Nine isolates appeared to be S3/S2, one S3/S1 and S1/S2 recombinant in analyzed 3Dpol region. This study revealed, through environmental monitoring, the introduction of SL PVs into the population associated with the routine use of OPV in Poland before the April 2016. Our findings demonstrate the usefulness of environmental surveillance in the overall polio eradication program.
Authors and Affiliations
Agnieszka Figas, Magdalena Wieczorek, Anna Żuk-Wasek, Bogumiła Litwińska
Keywords
Related Articles
- EP ID EP270370
- DOI 10.5604/01.3001.0011.6147
- Views 81
- Downloads 0
<strong>Biocontrol of Gray Mold Decay in Pear by Bacillus amyloliquefaciens Strain BA3 and its Effect on Postharvest Quality Parameters</strong>
The economic losses caused by postharvest fruits diseases have attracted global attention. Traditional chemical fungicide could not meet the need of humans. In recent years, microbial agent which has begun to take the pl...
<strong>Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in <em>Pseudomonas aeruginosa</em></strong>
[i]Pseudomonas aeruginosa[/i] is a leading human pathogen that causes serious infections at various tissues and organs leading to life threatening health problems and possible deadly outcomes. Resistance patterns vary wi...
<strong>Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by <em>Streptomyces</em> sp. 8812</strong>
The nutritional requirements and environmental conditions for a submerged culture of [i]Streptomyces[/i] sp. 8812 were determined. Batch and fed-batch [i]Streptomyces [/i]sp. 8812 fermentations were conducted to obtain h...
&lt;strong&gt;Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014&lt;/strong&gt;
A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were positive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) wer...
Influence of Pseudomonas and Bacillus Strains Isolated from Lolium perenne Rhizospheric Soil in Vojvodina (Serbia) on the Planth Growth and Soil Microbial Communities
The aim of this study was the isolation of Pseudomonas sp. and Bacillus sp. strains from rhizospheric soil and monitoring the impact of two isolates denoted as P12 (Pseudomonas sp.) and B1 (Bacillus sp.) on the parameter...