ISOLATION OF SECONDARY METABOLITE COMPOUNDS AND ANTIBACTERIAL ACTIVITIES TESTS FROM HEXANE EXTRACT OF STEM BARK MELOCHIA UMBELLATA (HOUTT) STAPF VAR. DEGRABRATA K
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 8
Abstract
Objectives: This research aims to determine the content of secondary metabolite compounds and antibacterial activity of stem bark extract Melochia umbellata (Houtt) Stapf var. degrabrata.Methods: M. umbellata stem bark was extracted by maceration using methanol solvent. Separation and purification were done by partitioning, fractionation with chromatography, and recrystallization. Antibacterial activity test of hexane extract and third isolate from the bark of M. umbellata was done by agar diffusion method against bacterium Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.Results: Phytochemical test showed that the hexane extracts of bark M. umbellata containing alkaloids and triterpenoids. Isolate D is a triterpenoid group compound, while FKa and FKb are steroid group compound. The hexane extract had the highest antibacterial activity against B. subtilis bacteria with inhibitory zone diameter 12.0 mm. Isolate D has a weak inhibitory effect on all test bacteria. The highest diameters inhibition zone of isolated FKa compound against B. subtilis and S. aureus bacteria was 18.0 mm and 13.0 mm, respectively, whereas, the highest diameter inhibition of zone FKb compound against B. subtilis bacteria with inhibitory zone was 12.0 mm.Conclusion: The FKa compound from the bark of M. umbellata has the potential to be antibacterial because the compound is able to inhibit bacterial growth with ˃14 mm obstacle zone, especially against B. subtilis bacteria.
Authors and Affiliations
Usman Usman, Muh Amir M, Nunuk Hariani Soekamto, Ahyar Ahmad, Maulidiyah Maulidiyah
Keywords
Related Articles
- EP ID EP567383
- DOI 10.22159/ajpcr.2018.v11i8.25464
- Views 113
- Downloads 0
INVESTIGATION OF CHEMICAL COMPOSITION FROM DRYOPTERIS CHOCHLEATA (D.DON) C. CHR. (DRYOPTERIDACEAE)
ABSTRACTObjective: The present study was for the determination of bioactive volatile compounds from chloroform and ethanolic extract.Methods: For present investigation of the samples were carried out by using Shimadzu Ma...
IS THERE TREATMENT EFFECT OF VINPOCETINE ON AUTONOMIC DYSFUNCTION IN RATS WITH ALZHEIMER
Alzheimer's disease (AD) shows less autonomic dysfunction. There was lack of response or prolongation of its latency in skin sympathetic response. Vinpocetine is a classic inhibitor of PDE1 activity. Vinpocetine tr...
Evaluation of phytochemical constituents, quantitative analysis and antimicrobial efficacy of potential herbs against selected microbes
 Objectives: The aim was to evaluate the physicochemical composition, phytochemical analysis and antimicrobial activity of plant extracts.Methods: The plant samples (Citrus reticulata, Camellia sinensis and Punica grana...
PHYTOCONSTITUENTS, PIGMENTS , G.C.M.S. ANALYSIS AND ALLELOPATHY EFFECT OF ALTERNANTHERA FICOIDEA (L.) P. BEAUV
Objective: The aim was to investigate phytoconstituents, composition of pigments, gas chromatography mass spectrometry (GCMS) and allelopathy of Alternanthera ficoidea (L.) P. Beauv.Methods: Qualitative phytochemical ana...
RATE OF MALIGNANT BREAST CANCER AMONG WOMEN WITH BREAST MASS ATTENDING ALDEWANIYAH TEACHING HOSPITALS: A CROSS-SECTIONAL STUDY
Objective: The problems of breast mass, pain, or discharge are extremely common in the Iraqi population, as well as, globally. However, the trend in most health-care institutions is that most breast lesions are of benign...