Isolation，identification and drug sensitivity of Erysipelothrix rhuriopathiae in a certain region of Guangdong
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 4
Abstract
"Erysipelothrix rhuriopathiae from the liver，kidney and spleen tissue of suspected E. rhuriopathiae swine in a certain region of Guangdong was isolated，then aquired dominant bacteria was identified by 16S rRNA primer gene sequence，the bacteria of E. rhuriopathiae was tested by toxicity test for SPF mice；ELISA method was used to test IgM and IgG in the serum of mice，to observe the pathological changes of liver and spleen；drug sensitive test was used to analyze the drug sensitive effect for five bacteria with nine kinds of antibiotic including penicillin， ampicillin，streptomycin，cefixime and cefotaxime etc. The results showed that five dominant bacteria were isolated and identified as E. rhuriopathiae by 16S rRNA and separated culture，the concentrations of IgM and IgG were obvious higher in the toxicity test group mice serum（ P<0.05），the cells of liver and spleen appeared different degree degeneration and necrosis in the infected mice. The results of drug sensitivity test showed that five bacteria were resistance to lincomycin，extrem sensitive to penicillin，ampicillin and cefotaxime，serious sensitive to cefalexin and medium sensitive to azithromycin and enrofloxacin."
Authors and Affiliations
Ying-jie LU
Keywords
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- EP ID EP598448
- DOI 10.16768/j.issn.1004-874X.2018.04.021
- Views 75
- Downloads 0
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