Isozyme analysis based genetic fidelity assessment of micropropagated banana plants
Journal Title: Journal of Applied and Natural Science - Year 2015, Vol 7, Issue 2
Abstract
Isozyme studies of micropropagated and mother plants of banana cvs. Matti, Ney Poovan, Kechulepa, Dwarf Cavendish, Malbhog, Champa, B.B. Battisa and FHIA-1 were done to test their genetic fidelity. The banding patterns as revealed by electrophoretic variations were evaluated with respect to isozymes of acid phosphatase, catalase, esterase and peroxidase as markers. The genetic fidelity of micropropagated plants and the relationship of the different cultivars were determined by dendrogram using numerical taxonomy and multivariate analysis system (NTSYS). A clustered dendrogram was prepared by unweighted pair group method using averages (UPGAMA) method. At 87% similarity, the micropropagated and mother plants were clustered in four groups reflecting their genomic constitution. Cvs. Matti (AA) and Dwarf Cavendish (AAA) with similar ‘A’ genome were categorized in Cluster I. Cluster II comprised of cvs. Ney Poovan (AB), B.B. Battisa (ABB) and FHIA-1(AAAB) with genomic constitution of both ‘A’ and ‘B’ type. Cvs. Champa (AAB) and Malbhog (AAB) with similar genome were grouped in Cluster III. Cluster IV contained the cv. Kechulepa (BB) having only ‘B’ genome. However, there was no somaclonal variation among the micropropagated plants and they showed 100% genetic similarity. Thus, the isozyme studies could be a reliable marker for testing the genetic fidelity of micropropagated plants and for evaluating the diversity among the banana germplasm.
Authors and Affiliations
Sugandh Suman, Ranju Kumar, V. K. Sharma, Harsh Kumar
Keywords
Related Articles
- EP ID EP283218
- DOI 10.31018/jans.v7i2.648
- Views 86
- Downloads 0
Documentation of ethno-medicinal practices: A case study on tribal forest fringe dwellers of Terai West Bengal in India
The ethno-medicinal practices were documented which were being used by tribal population dwelling forest fringe areas of Terai zone of West Bengal, India on under exploited, non conventional, traditional and indigenous p...
Response of weed management practices on the Productivity of urdbean (Vigna mungo L. Hepper)
A field Investigation was carried out to evaluate the effect of hand weedings and herbicides on the weed flora, growth and yield of urdbean (Vigna mungo). The experiment was conducted during kharif season 2011 in loamy s...
Effect of different factors on in vitro growth and shoot proliferation of guava (Psidium guajava L.) cv. Allahabad Safeda
In the present experiment an attempt has been made to optimize the effect of different medium, levels of sucrose, pH, adenine sulphate and light intensity for culture establishment and shoot proliferation in guava using...
Efficacy of dormancy breaking methods in paddy genotypes
Paddy plays a pivotal role in Indian agriculture. It also possesses dormancy which needs to be studied thoroughly. Duration of dormancy usually ranges from 7 to 35 days. Environmental conditions that facilitate after-rip...
Comparison of floor bacterial load in different types of rabbit cages
Rabbits are housed on various types of cages including single tier, multi- tier cages etc. The effect of housing system on the floor bacterial load is variable, and its effect on ani-mal production is limited. An attempt...