ISSR marker based DNA profiling studies in Rauwolfia species
Journal Title: Annals of Plant Sciences - Year 2018, Vol 7, Issue 5
Abstract
In this present study, a protocol was developed for the DNA profiling of medicinally important Rauwolfia species (05 species) by ISSR-PCR method. Initially genomic DNA was isolated from the fresh leaves (1 gram) of 24 months old Rauwolfia tetraphylla, R.serpentina, R.pentaphylla, R.vomitaria and R.micrantha. The content of genomic DNA isolated from 1 gram of fresh leaves of these five species ranges from 1.6 to 3.4 µg. Out of six inter simple sequence repeats (ISSR) primers used, HY3 primer has given the good amplification with distinct bands which were reproducible. The range of DNA bands produced by the HY3 primer were between 300 bp to 1500 bp among the five species. A dendrogram was constructed on the basis of similarity matrix data by unweighted pair group method with arithmetic average (UPGMA) cluster analysis using indigenous software. Average genetic similarity generated by HY3 has revealed 38% similarity between species of Sub cluster-A and species of Sub cluster-B which is consistent with their geographical distribution i.e. Sub cluster-A species R.tetraphylla and R.serpentina inhabit peninsular India, Sub cluster- B species R.micrantha and R. vomitoria inhabit the Himalayan foot hills. The developed protocol of DNA profiling in Rauwolfia species by ISSR-PCR method could be used efficiently for the differentiation and identification of the Rauwolfia species at molecular level.
Authors and Affiliations
Gulab Khan Rohela, Prasad Bylla, Sreenu Pendli, Korra Rajender, Christopher Reuben
Keywords
Related Articles
- EP ID EP304952
- DOI 10.21746/aps.2018.7.5.13
- Views 127
- Downloads 0
Angiotensin-I converting enzyme (ACE-I) inhibitory and antiproliferative potential of chickpea seed protein hydrolysate
Chickpea seeds are the preferred source of proteins possessing health care functions in countries across the world. In the present investigation, the chickpea seed protein subjected to enzymatic hydrolysis produced bioac...
Diversity of Coprinus species in North-Eastern part of Uttar Pradesh, India.
Gorakhpur is an ‘oriental region’ providing a profusion of habitats, which features diverse biota with a high level of endemism. The region is an important sub-centre for the origin of mushrooms, the most important among...
Effect of veterinary antibiotics on the seed germination of indica rice varieties
The effect of three antibiotics with different action modes (namely, tetracycline, amoxicillin and trimethoprim) on three different rice (Oryza sativa L.) varieties. Each antibiotic test had 6 treatments with tetracyclin...
Allergic evaluation of Roystonea regia (Kunth.) O. F. Cook. among Keralites, India.
Aerobiological studies conducted in Kerala (India), revealed the incidence of pollen grains of Roystonea regia (Kunth.) O.F.Cook. belonging to the family Aracaceae as a common airborne pollen member. Previous clinical st...
Micropropagation of traditional medicinal plant Ceropegia juncea
Ceropegia species which possess wide medicinal properties are being used in different traditional medicinal systems that are used by tribal people for curing different ailments. Ceropegia juncea was reported to be the so...