Kainic Acid and MPP+ Induce Upregulation of GLT-1 in Neuroblastoma and Glia Cells
Journal Title: Bezmiâlem Science - Year 2021, Vol 9, Issue 2
Abstract
Objective: Glutamate is the major excitatory transmitter in the brain. The excessive glutamate will lead to excitotoxicity. Glutamate transporter-1 (GLT-1) is the major transporter that performs 95% of the glutamate clearance contributing to normal neuronal function and preventing excitotoxicity. In this study, we investigated the effect of two toxins, kainic acid and MPP+ (1-methyl-4- phenylpyridinium), on GLT-1 expression and excitotoxicity in neuroblastoma and glia cells (immortalized human astrocytes). Methods: We treated neuroblastoma and glia cells with kainic acid and MPP+, applied MTT assay to measure the cell viability. We identified the mRNA and protein levels of GLT-1 and also analyzed released glutamate levels using glutamate assay. Results: The mRNA level of GLT-1 increased in neuroblastoma cells as a result of kainic acid or MPP+ treatment while the protein expression of GLT-1 increased in glia cells after the treatment with MPP+. Excess glutamate was found to be decreased after 12 h MPP+ treatment. However, this decrease was no more prominent with further MPP+ treatment. Conclusion: Our results show that GLT-1 levels are elevated as a result of kainic acid or MPP+ treatment as a survival mechanism to prevent excitotoxicity.
Authors and Affiliations
Rabia UYARICI, Ceylan AK, Gizem DÖNMEZ YALÇIN
Keywords
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- EP ID EP693763
- DOI 10.14235/bas.galenos.2020.4032
- Views 99
- Downloads 0
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