Keratitis induced by Allovahlkampfia spelaea in experminental rat model: a trial for treatment with ellagic acid
Journal Title: European Journal of Biological Research - Year 2016, Vol 6, Issue 4
Abstract
Members of family Acanthamoebidae and Vahlkampfiidae are amphizoic, occurring as human parasite causing many diseases. This study was aims to evaluate the efficacy of ellagic acid (EA) to ameloriate the histological changes in cornea and the changes in oxidative stress markers in different organs of rats infected with Allovahlkampfia spelaea. Thirty rats were intraocular infected with trophozites of A. spelaea. Fourteen days later, rats divided into four groups, treated with chlorhexidine (CHX), EA, EA plus CHX, and lubricant eye drops for 14 days, respectively. All eyes were examined clinically then rats were killed and their corneas, brain, liver, ling, kidney and spleen were excised and used for histological and biochemicals evaluation. Eyes from A. spelaea infected rats showed corneal ulcer with disruption of corneal layer, congestion and infilteration of the inflammatory cell in stromal layer. However, the cornea of CHX, EA, and the combination of CHX and EA treated rats showed hyperplasia in the epithelial layer of cornea, hyperplasia in the epithelial layer of cornea with stromal vascularization and epithelial hyperplasia, stromal vascularization with fibroblast cell activation, respectively. The activity of acetylchoinestase in the brain and the markers of oxidative stress in the brain, lung, liver, kidney and spleen were altered in infected rats with A. spelaea and restored by treatment with CHX, EA, or the combination of CHX and EA. In conclusion infection with A. spelaea induced keratitis and biochemical changes in organs of rats, these changes were ameloriated by the treatment with CHX or EA or the combination of both with the priority of the later one.
Authors and Affiliations
Enas A. M. Huseein, Fatama E. S. Anwer, Gamal H. Abed, Sary Kh. Abdel-Gahfar, Hossam El-Din Mohamed Omar
Keywords
Related Articles
- EP ID EP230400
- DOI 10.5281/zenodo.192465
- Views 134
- Downloads 0
Toxicological investigation and anti-reproductive effect of phyto-molluscicide against harmful aquatic snail
In this study attempt was made by using active component Taraxerol isolated from medicinal plant of India, Codiaeum variegatum (Family: Euphorbiaceae) to control harmful disease schistosomiasis in domestic animals caused...
Diversity of phylloplane microflora in certain tea cultivars of Assam, North-East India
The phylloplane is considered as the hostile environment for microbial growth. Phylloplane microflora plays important role affecting the plant-microbe interactions in leaf surface and thereby contribute significantly for...
Immunosuppressive and cytotoxic potential of aqueous leaves extract of Thlaspi arvense
Medicinal plants are traditionally used in Asian countries for treatment of various intracellular as well as extracellular infections. The present study was undertaken to investigate Thlaspi arvense for determining its i...
Plant litter decomposition: drivers insight to the ecological process
Decomposition of litter is a function of various interrelated variables; both biotic factors and abiotic factors. Litter decomposition acts like a natural fertilizer, plays a prime role in maintaining the productivity an...
Allometric models for branch biomass production: assessment of rapid growth trees for bio-energy in Northern Iran
The increase of atmosphere carbon dioxide (CO2) as a result of burning fossil fuel is becoming a global issue. Biomass fuels are an environmental friendly alternative for energy supply. Among the sources of biomass, fore...