L-Phenylalanine and L-Tyrosine Catabolism by Thermophilic Geobacillus stearothermophilus
Journal Title: Biotechnology Journal International - Year 2013, Vol 3, Issue 4
Abstract
Aims: This study investigated the potential of soil thermophile Geobacillus stearothermophilus for the biotransformation of phenylalanine and tyrosine. Study Design: G. Stearothermopilus grows well at 65ºC and has a good potential for transformation and biodegradation of many compounds including steroids, bile acids, tryptophan and other compounds. In this study G. stearothermophilus was harvested at mid-log phase at 65ºC, on tryptone yeast extract (TYE) medium. Cells were collected by centrifugation under aseptic conditions, washed with sterile water and suspended in phosphate buffer with phenylalanine or tyrosine as sole source of carbon at 65ºC. Metabolic parameters were optimized for optimal growth of the organism utilizing aromatic amino acids as an exclusive source of carbon. Methodology: The amino acid metabolites were exhaustively extracted with methanol from freeze dried broth. The concentrated pooled extracts were analyzed by thin layer chromatography (TLC) using polar solvent systems and purification of the extracts was achieved on preparative tlc plates and GC separations. The molecular structures of purified metabolites were established through spectral data. Results: Sixteen metabolites of phenylalainine and seventeen metabolites of tyrosine were identified in this study. Tyrosine metabolism extensively produced melanin pigments that caused hitches in the purification of tyrosine metabolites. Tyr metabolites were analyzed in cells cultured for short time. Conclusion: Our data suggest that G. stearothermophilus has a good potential to metabolize aromatic amino acids yielding hydroxylated, deaminated, decarboxylated and many other products. Oxidative metabolism of phenylalanine and tyrosine by a thermophilic G. stearothermophilus is being reported for the first time.
Authors and Affiliations
M. Afzal, S. Al-Awadhi
Keywords
Related Articles
- EP ID EP237149
- DOI 10.9734/BBJ/2013/4516
- Views 143
- Downloads 0
Phyletic Evolution of Ophiocordyceps sinensis through Interkingdom Host Colonization between Plants and Insects
Ophiocordyceps sinensis, an entomogenous fungus parasitic in the larvae of ghost moths (Lepidoptera), has always been used as one of the most valued Traditional Chinese Medicines throughout the Orient World. However, O....
The Effect of Drying and Salting on the Nutrient Composition and Organoleptic Properties of Talinum triangulare Leaves
Aims: The present study was undertaken to determine the effects of drying and salting on the nutrient content and sensory properties of Talinum triangulare leaves. Study Design: The methods of drying and salting were: o...
Ex-situ Conservation of the Rare and Threatened Medicinal Climber Corallocarpus epigaeus Rottler through In vitro Regeneration Method
Aims: To develop in vitro propagation and callus induction protocol for regeneration and conservation of the rare and threatened medicinal climber, Corallocarpus epigaeus, which is widely acclaimed for high medicinal val...
Use of Gene Specific Universal Primers for Isolation of DNA Sequences Encoding Laccase Enzyme from a Wild Isolate of Schizophyllum commune
Laccase enzymes plays a vital role in innumerable biotechnological applications and hence their large scale production has stimulated considerable research. In the present study, degenerate universal primer pairs were em...
Phosphate-Solubilizing Bacteria in the Rhizosphere of Some Cultivated Legumes from Meknes Region, Morocco
Aims: Through the National Botanical Research Institute's phosphate growth medium (NBRIP) and 16S rDNA sequence analysis were used to isolate and identify the bacterial groups that actively solubilized phosphates in vitr...