Laboratory Identification of Metallo-beta-lactamase Producing Clinical Isolates of Pseudomonas aeruginosa: An Assessment of Different Phenotypic Methods
Journal Title: Journal of Krishna Institute of Medical Sciences University - Year 2018, Vol 7, Issue 3
Abstract
Background: Multidrug Resistant (MDR) Pseudomonas aeruginosa, an emerging superbug causing a wide spectrum of nosocomial infections. Carbapenem resistance among P. aeruginosa isolates is of grave concern and is mainly due to production of Metallobeta-lactamase (MBL) enzymes. Aim and Objectives: To find out the prevalence of MBL producing P. aeruginosa isolates from various clinical samples and to evaluate the efficacy of different phenotypic tests employed in vitro for their detection. Material and Methods:Atotal of 358 P. aeruginosa strains obtained from different clinical samples were subjected to antimicrobial susceptibility testing by Kirby-Bauer's Disc Diffusion method. All the imipenem resistant isolates were further tested by Modified Hodge Test (MHT) to detect carbapenem resistance. MBL production was tested by screening with Combined Disc Test (CDT) and Double Disc Synergy Test (DDST). MBL production was confirmed by MBL Etest (Ab BioDisk). Results: Among 358 strains of P. aeruginosa recovered, 114 isolates showed resistance to imipenem. E test demonstrated 73 out of 114 isolates as MBL producers. Of MHT, CDT and DDST tests performed, DDST showed high sensitivity and specificity. Conclusion: DDST can be suggested as an economical option in place of expensive E test for routine screening of MDR P. aeruginosa isolates for MBL production in a clinical laboratory; which is crucial for planning better management protocols.
Authors and Affiliations
Aruna Siddabathuni
Keywords
Related Articles
- EP ID EP354738
- DOI -
- Views 99
- Downloads 0
An Ectopic Thyroid Tissue - Parapharyngeal Region: A Rare Entity - Case Report
An ectopic thyroid encompasses a rare entity due to the defective embryological development and descent of the gland originating from the primitive foregut's floor to its terminal pre-tracheal location. Its prevalence is...
Primary Cutaneous Nocardiosis of Axillary Region: A Case Report
Background: Nocardiosis is an uncommon but world-wide infection caused by several species of soil-borne aerobic bacteria belonging to the genus Nocardia. Primary cutaneous nocardiosis (PCN) is an uncommon entity. It usua...
Ophthalmological Pointers to Hepatic Disorders in Children
The value of ophthalmological examination is often overlooked when we deal with disorders of hepatobiliary system. There is a wide spectrum of genetic, metabolic and other acquired disorders in infants and children that...
Opportunities for Cervical Cancer Prevention in India
Occupational Health Hazards of Women Working in Brick Kiln and Construction Industry
Background: In brick kiln and construction industry the exposure to carbon monoxide and silica dust is the most common occupational hazard to the workers in these industries. A study on occupational health hazards of...