Laboratory toxicity evaluation of Diflubenzuron, a chitin-synthesis inhibitor, against Anopheles darlingi (Diptera, Culicidae)
Journal Title: Journal of Research in Biology - Year 2011, Vol 1, Issue 6
Abstract
We evaluated Diflubenzuron toxicity against larvae and pupae of Anopheles darlingi. A series of bioassays were developed to assess the lethal concentrations LC50 and LC90 to larvae after two exposure periods (24 and 48 hours), and to evaluate its toxicity to 20-minute- and 24-hour-old pupae. The LC50 and LC90 obtained were 0.006 and 0.013 ppm, respectively. For concentrations of 0.01 to 0.1 ppm, a mortality of 100% was observed, mainly during the larval stage. With concentrations between 0.009 and 0.001 ppm, mortality varied from 1.7% to 25.8% in the larval stage; from 21.7% to 44.2% in the pupal stage; and from 15.0% to 4.2% in adults. No differentiation in emergence inhibition was observed between the exposure periods of 24 (82.0%) and 48 hours (86.7%). These results revealed that the minimum exposure period of 24 hours significantly inhibits emergence. The 20-minute-old pupae were more susceptible than the 24-hour-old ones (61.1% emergence inhibition, against 93.0% of the former). Diflubenzuron toxicity to pupae indicates that this insecticide may also act by contact. These results showed that A. darlingi larvae and pupae are susceptible to the inhibitory action of Diflubenzuron
Authors and Affiliations
Costa FM, Tadei WP
Keywords
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