Lack of Association between Endothelial Nitric Oxide Synthase Gene Polymorphisms and Coronary Artery Disease among Egyptian Population
Journal Title: International Journal of Biochemistry Research & Review - Year 2016, Vol 11, Issue 2
Abstract
Controversial results regarding the association of e NOS gene polymorphisms with Coronary Artery Disease (CAD) have been reported up to now, there has been conflicting data regarding the association between two clinically relevant polymorphisms (T-786C) in the promoter region and intron 4 variable number of 27-bp tandem repeats (VNTR) of the eNOS gene and coronary artery disease (CAD). The present study was undertaken to investigate association of these two eNOS gene polymorphisms with susceptibility to CAD in Egyptian population. A total of 80 patients with CAD and 40 healthy controls were included in this study. CAD patients were divided according to their body mass index (BMI) limits into 2 main groups: normal weigh group (BMI: 18.5–24.9, n=40) and abnormal weight group (BMI≥25, n= 40), the last group was further sub-divided into overweight group (BMI: 25.0–29.9, n= 20) and obese group (BMI≥ 30, n= 20). The T-786C and intron 4 VNTR polymorphisms were analyzed by polymerase chain reaction (PCR) using specific primers. The T-786C polymorphism frequencies for T/T, T/C and C/C genotypes were 27.5%, 28.75%, 43.75% respectively in the CAD patients, and 35%, 37.5%, 27.5% in the control subjects, while allele frequencies for C, T were 58.12%, 41.87% in the CAD patients and 46.25%, 53.75%, in the control group. No significant differences were observed in genotype frequencies between CAD patient group when compared to control group, or between them according to allele frequency. Also, there was no significant differences in the genotype distribution and allele frequencies of T786-C polymorphism among the overweight and obese cases compared to normal weight subjects. The genotype frequencies for eNOS b/b, a/b, a/a were 45%, 50% and 5% in the CAD patients, and 35%, 65% and 0% in the control subjects, with no significant difference between the two groups. Also, there were no significant difference in the allele frequencies between the CAD patients (a: b = 30%:70%), and controls (a: b = 32.5%:67.5%) as well as in the distribution of genotype and alleles frequencies of intron 4 VNTR of eNOS gene when overweight and obese patient groups were compared with the normal weight patient group. Thus the present study suggested that the two eNOS gene polymorphisms (the eNOS T-786C and intron 4 VNTR polymorphisms) were unlikely to be major genetic susceptibility factors for CAD in the Egyptian population even after classification of the CAD patients according to their BMI. Further studies with larger sample size are required to be done to confirm these findings.
Authors and Affiliations
Afaf M. El Saied, Azza M. El Wakf, Rehab Elmougy, AfafAbd El Hafez, Sahar M. Hussien
Keywords
Related Articles
- EP ID EP351182
- DOI 10.9734/IJBCRR/2016/23818
- Views 121
- Downloads 0
Calcium Chloride Can Influence the Stability Flexibility Complementarity and Consequently, Activity of Hydrolases: A Case Study on Porcine Alpha Amylase
Aims: The specific aims of the research were i) to investigate the effects of extra “load” of calcium chloride at different temperatures and ethanol on the specific activity of porcine alpha amylase (PPA), ii) characteri...
Effects of Fermentation on Nutritional and Microbial Qualities of Ash Neutralized Roselle (Hibiscus sabdariffa L.)
The study was conducted to investigate the effect of fermentation on the nutritional and microbial qualities of Roselle calyces from two States in Nigeria. Roselle calyx was fermented naturally (FN), fermented with cocon...
Multi-residue Pesticides in Conventional and Organic Orange
A monitoring project for pesticide residues in orange samples in Al-Tamer vegetables market, Riyadh was conducted. A total of 144 samples of organic and non-organic orange were collected according to the Codex Alimentari...
Study of Thyroid Dysfunction in Association with Infertility
Aims: To evaluate the relation of female infertility to thyroid dysfunction. Study Design: This is a descriptive, hospital-based study. Place and Duration of Study: The present study was carried out in the department of...
Non-targeted Metabolomic Profiling of Maize Landraces (Zea mays L.) Combined with Chemometric Tools
Grain samples of maize landraces were collected and subjected to Fourier Transform Infrared spectroscopy (FTIR) analysis combined with chemometric tools in other to discriminate them regarding the chemical composition. P...