Langmuir-Blodgett Protein Multilayer Nanofilms by XFEL
Journal Title: NanoWorld Journal - Year 2018, Vol 4, Issue 4
Abstract
Serial femtosecond crystallography (SFX) at X-ray free electron lasers (XFELs) has created many new opportunities for protein crystallography, including radiation damage mitigation and the study of dynamics at room temperature. This field is rapidly evolving, requiring new methods of macromolecule organization into diffracting arrays, since current methods of sample preparation and delivery are often the bottleneck which limits productivity. We propose here the Langmuir- Blodgett (LB) protein nanofilm technology as a novel approach for direct “on chip” protein molecules organization into the 3D ordered diffracting arrays. The combination of this advanced technology with the XFEL for fixed target SFX has the potential to become an important tool for the structure determination of proteins that are difficult to crystallize, such as membrane proteins of life science interest and with pharmaceutical industry impact.
Authors and Affiliations
Eugenia Pechkova, Claudio Nicolini
Keywords
Related Articles
- EP ID EP519983
- DOI 10.17756/nwj.2018-060
- Views 95
- Downloads 0
Acknowledgement of Manuscript Reviewers NWJ Volume-2 (2016)
The Editors of NanoWorld Journal (NWJ) would like to express their tremendous gratitude to all those individuals who participated in the peer review process during the preparation of this Volume-2 (2016). Many reviewers...
Proceedings of the 2nd NanoWorld Conference in Boston (NWC-2017). Part VI: Conclusions of World Peace Forum and of NWC 2017
In the last few years the problems of humanity are growing in all sectors of the economy, from energy to environment and disasters induced by nuclear reactors, from cancer to hardware and space such as asteroids. More th...
Proceedings of the 2nd NanoWorld Conference in Boston (NWC-2017). Part III: Featured Presentations
The session on Nano-electronics was initiated by Prof. Xiaosheng Fang, Fudan University, China and Session talk were delivered by renowned experts -Prof. Seongil Im from Yonsei University, S. Korea on the “Two Dimensiona...
Epitaxial Growth of Ruthenium Dioxides on Ru(0001) Surface
The epitaxial growth of the RuO2 clusters on Ru(0001) electrode formed by electrooxidation in HClO4 solution has been studied by ex-situ reflection high energy electron diffraction (RHEED), low energy electron diffractio...
How Bibliometric Indicators Should Be Used to Assess Excellence in Science and Technology
In order to identify the best standards for the assessment of world-wide scientific performances at the level of both individuals and institutions, we have carried out a comparative analysis of the relative scientific an...