Linoleic acid effect on PGF2a and PGE2 prostaglandin production in endometrial cells
Journal Title: Revista MVZ Cordoba - Year 2013, Vol 18, Issue 2
Abstract
Objective: Evaluate the in vitro effect of AL on the production of PGF2a and PGE2 in bovine epithelial endometrial cells (CEEP). Materials and methods. Isolated CEEP were cultured and supplemented with AL at different concentrations (1μM, 10μM, 100μM), oxytocin (OT) (0.1 μM) and bovine interferon (BINT-τ) (50 ng / ml). PGF2a and PGE2 were quantified as response variables at different times. In the control, the concentration of PGF2a in the supernatant increased over time and was 1.2 times greater than the production of PGE2. Results. AL reduced the concentration of PGF2a (p <0.05) in the supernatant, and did not affect (p> 0.05) the production of PGE2. The combined effect of LA and OT in the production of PGF2a is different in time, LA partially inhibited the stimulatory effect of OT in the production of PGE2, the joint effect of LA and BINT-τ increased (p<0.05) when this inhibition at 24h. Conclusions. LA alone negatively affects the concentration in the supernatant of PGE2 in time. Regarding PGE2 , LA alone does not affect the concentration in the supernatant.
Authors and Affiliations
Yasser Lenis S, M. Sc *| Universidad de Antioquia, Facultad de Ciencias Agrarias, Escuela de Medicina Veterinaria, Grupo de investigación CENTAURO. Medellín, Colombia.Correspondencia: yaserudea@gmail.com, Martha Olivera A, Ph. D| Universidad de Antioquia, Facultad de Ciencias Agrarias, Escuela de Medicina Veterinaria, Laboratorio Syngamia, Grupo de investigación BIOGÉNESIS. Medellín, Colombia., Ariel Tarazona M, M. Sc| 3Universidad Nacional de Colombia, Facultad de Ciencias Agrarias, Departamento de producción Animal. Medellín, Colombia.
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