Linoleic acid effect on PGF2a and PGE2 prostaglandin production in endometrial cells
Journal Title: Revista MVZ Cordoba - Year 2013, Vol 18, Issue 2
Abstract
Objective: Evaluate the in vitro effect of AL on the production of PGF2a and PGE2 in bovine epithelial endometrial cells (CEEP). Materials and methods. Isolated CEEP were cultured and supplemented with AL at different concentrations (1μM, 10μM, 100μM), oxytocin (OT) (0.1 μM) and bovine interferon (BINT-τ) (50 ng / ml). PGF2a and PGE2 were quantified as response variables at different times. In the control, the concentration of PGF2a in the supernatant increased over time and was 1.2 times greater than the production of PGE2. Results. AL reduced the concentration of PGF2a (p <0.05) in the supernatant, and did not affect (p> 0.05) the production of PGE2. The combined effect of LA and OT in the production of PGF2a is different in time, LA partially inhibited the stimulatory effect of OT in the production of PGE2, the joint effect of LA and BINT-τ increased (p<0.05) when this inhibition at 24h. Conclusions. LA alone negatively affects the concentration in the supernatant of PGE2 in time. Regarding PGE2 , LA alone does not affect the concentration in the supernatant.
Authors and Affiliations
Yasser Lenis S, M. Sc *| Universidad de Antioquia, Facultad de Ciencias Agrarias, Escuela de Medicina Veterinaria, Grupo de investigación CENTAURO. Medellín, Colombia.Correspondencia: yaserudea@gmail.com, Martha Olivera A, Ph. D| Universidad de Antioquia, Facultad de Ciencias Agrarias, Escuela de Medicina Veterinaria, Laboratorio Syngamia, Grupo de investigación BIOGÉNESIS. Medellín, Colombia., Ariel Tarazona M, M. Sc| 3Universidad Nacional de Colombia, Facultad de Ciencias Agrarias, Departamento de producción Animal. Medellín, Colombia.
Keywords
Related Articles
- EP ID EP3318
- DOI -
- Views 398
- Downloads 14
Physiology of acid-base balance in bovines with diarrhea backgrounds from Monteria, Colombia
Objective. Evaluate the acid-base balance (ABB) in bovines with diarrheic backgrounds in four areas of Montería, Colombia. Materials and methods. From a total of 300 pregnant cows, 60 were selected with their newborns. A...
Selenium metabolic status and response to supplementation in grazing Chilean-Criollo horses
Objective. Two experiments were conducted to determine the selenium status in grazing Chilean-Criollo horses and evaluate their response to a Na2SeO3 supplement. Material and methods. Exp.1The blood activity of glutathio...
SEROPREVALENCIA OF BOVINE LEUKEMIA VIRUS IN ANIMALS WITH REPRODUCTIVE PROBLEMS IN MONTERIA
Objective. To determine the seroprevalence of bovine leukemia virus (BLV) in animals with reproductive problems. Materials and methods. Blood samples were collected from 137 cows with a history of infertility, belonging...
Corn grain processing on the kinetics of in vitro dry matter degradation
Objective. Determine the effect of processing corn grain on the kinetics of degradation and in vitro fermentation of dry matter. Materials and methods. There were five treatments, each one consisting of a mixture of two...
ENVIROMENTAL AND GENETIC ASPECTS RELATED WITH CALVING INTERVAL IN ROMOSINUANO BREED
Objective. To study the environmental and genetic factors that influence on the calving interval of romosinuano females bovines. Materials and methods. 1.911 data were analyzed retrospectively the interval calving of r...