Lipidic Systems for In Vivo siRNA Delivery

Journal Title: The AAPS Journal - Year 2009, Vol 11, Issue 4

Abstract

The ability of small-interfering RNA (siRNA) to silence specific target genes not only offers a tool to study gene function but also represents a novel approach for the treatment of various human diseases. Its clinical use, however, has been severely hampered by the lack of delivery of these molecules to target cell populations in vivo due to their instability, inefficient cell entry, and poor pharmacokinetic profile. Various delivery vectors including liposomes, polymers, and nanoparticles have thus been developed in order to circumvent these problems. This review presents a comprehensive overview of the barriers and recent progress for both local and systemic delivery of therapeutic siRNA using lipidic vectors. Different strategies for formulating these siRNA-loaded lipid particles as well as the general concern about their safe use in vivo will also be discussed. Finally, current advances in the targeted delivery of siRNA and their impacts on the field of RNA interference (RNAi)-based therapy will be presented.

Authors and Affiliations

Sherry Y. Wu, Nigel A. J. McMillan

Keywords

Related Articles

Ligand Binding Assays in the 21st Century Laboratory—A Call for Change

In June 2009, a group of ligand binding assay experts met in Seattle, WA to discuss the need for greater efficiency in bioanalytical laboratories. The central premise was increased utilization of technological innovation...

Covariate Pharmacokinetic Model Building in Oncology and its Potential Clinical Relevance

When modeling pharmacokinetic (PK) data, identifying covariates is important in explaining interindividual variability, and thus increasing the predictive value of the model. Nonlinear mixed-effects modeling with stepwis...

Summary Workshop Report: Facilitating Oral Product Development and Reducing Regulatory Burden Through Novel Approaches to Assess Bioavailability/Bioequivalence

This summary workshop report highlights presentations and over-arching themes from an October 2011 workshop. Discussions focused on best practices in the application of biopharmaceutics in oral drug product development a...

Points to Consider when Establishing Drug Product Specifications for Parenteral Microspheres

Drug product specifications are a critical element of a good control strategy. Parenteral microsphere products are complex dosage forms, requiring careful development of test methods and acceptance criteria for the speci...

Brain tissue lipidomics: Direct probing using matrix-assisted laser desorption/ionization mass spectrometry

Lipidomics is the new frontier in biomolecular structural studies. Not only are lipids the main components in membranes that define the contours of the cell and its organelles, but they are also used for storage. Lipids...

Download PDF file
  • EP ID EP681505
  • DOI  10.1208/s12248-009-9140-1
  • Views 69
  • Downloads 0

How To Cite

Sherry Y. Wu, Nigel A. J. McMillan (2009). Lipidic Systems for In Vivo siRNA Delivery. The AAPS Journal, 11(4), -. https://europub.co.uk/articles/-A-681505