Lipopolysaccharide accelerates fine particulate matter-induced cell apoptosis in human lung bronchial epithelial cells
Journal Title: International Journal of Occupational Medicine and Environmental Health - Year 2018, Vol 31, Issue 2
Abstract
Objectives: The aim of the study has been to investigate the effect of the Standard Reference Material of fine particulate matter (SRM 2786) on cytotoxicity and apoptosis in human lung bronchial epithelial cells (16HBE cells). Whether the lipopolysaccharide (LPS)-induced inflammation could further accelerate cell apoptosis induced by SRM 2786 stimulation has also been determined. Material and Methods: 16HBE cells were exposed to various doses of SRM 2786 with or without LPS. The following parameters: cytotoxicity, apoptotic rate, Bax/Bcl-2 expression, nitric oxide (NO) production, and reactive oxygen species (ROS) generation were measured. Results: The results have shown that SRM 2786 induces cell damage and apoptosis of 16HBE cells as demonstrated by significant decrease in expression of Bcl-2 and increase in expression of Bax. When compared with the control cells, the apoptotic rate of cells treated by 500 μg/ml of SRM 2786 increased from 2.43±0.21% to 43.96±2.95% (p < 0.01). Further, there was an elevated production of NO and ROS post SRM 2786 treatment. The level of NO in cells treated with 500 μg/ml of SRM 2786 was 18.33±1.02 μmol/l whereas that of control cells was 1.58±0.31 μmol/l (p < 0.01). When compared with the control group, the level of intracellular ROS increased by 24% after treatment with 500 μg/ml of SRM 2786 (p < 0.05). In addition, LPS pre-treatment may accelerate cell apoptosis by increasing generation of NO and ROS followed by SRM 2786 stimulation. When compared to cells treated with 125 μg/ml of SRM 2786 alone, the levels of NO and ROS in cells pretreated with LPS increased by 28% and 11.6%, respectively (p < 0.05), and the apoptotic rate increased from 34.62±4.44% to 54.11±3.34% (p < 0.01). Conclusions: These findings have suggested that in vitro exposure to SRM 2786 could induce 16HBE cells apoptosis probably by means of the mechanism involving the generation of free radicals, while the degree of apoptosis would be further aggravated under inflammation condition. Int J Occup Med Environ Health 2018;31(2):173–183
Authors and Affiliations
Qin Ru, Qi Xiong, Lin Chen, Xiang Tian, Kai Yue, Baomiao Ma, Lu Liu, Rihui Wu, Congyue Xu, Mingshan Pi, Chaoying Li
Keywords
Related Articles
- EP ID EP272391
- DOI 10.13075/ijomeh.1896.00527
- Views 83
- Downloads 0
The factorial structure of job-related affective well-being: Polish adaptation of the Warr’s measure
Objectives: The first aim of the study reported in this article was to test the factorial structure of job-related affect in a Polish sample. The second aim was to develop the Polish adaptation of the Warr’s job-related...
Authors’ response (December 8, 2017) to the letter to the Editor concerning the paper “Occupational exposure to radon for underground tourist routes in Poland: Doses to lung and the risk of developing lung cancer”
Refers to: Mortazavi JSM, Bevelacqua JJ, Fornalski KW, Pennington CW, Welsh J, Janiak MK, et al. Letter to the editor (August 24, 2017) concerning the paper “Occupational exposure to radon for underground tourist routes...
POLISH BIBLIOGRAPHY OF OCCUPATIONAL MEDICINE, 2008
Rare occupational cause of nasal septum perforation: Nickel exposure
Many etiologies are held accountable for nasal septum perforations. Topical nasal drug usage, previous surgeries, trauma, nose picking, squamous cell carcinoma, some rheumatological disorders such as granulomatosis with...
Prevalence of carpal tunnel syndrome and its correlation with pain amongst female hairdressers
Objectives: Carpal tunnel syndrome (CTS) may develop with repetitive and forced movements of the hands and wrists. In this study, we have aimed to evaluate whether the frequency of CTS is increased amongst female hairdre...