Lipoprotein (a) Internalization by RAW 264.7 Cells is Associated with Morphological Changes and Accumulation of Lipids Detectable by Two-photon Scanning Microscopy
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 17, Issue 7
Abstract
Lipoprotein (a) [Lp(a)] is an independent cardiovascular risk factor and its pathogenic mechanism is not completely clear. Lp(a) has been detected in atherosclerotic plaques and macrophages are one of the major cell types involved in atherogenesis. In order to characterize internalization of Lp(a) by RAW 264.7 cells, an established model of mouse macrophages, cells were treated with Lp(a) samples purified from plasma by affinity chromatography, and evaluated by western blotting. By 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, a tetrazolium salt, assay, Lp(a) was found to be non-cytotoxic for the cells at all the concentrations tested (0.0165-1.65 mg/ml). An ELISA performed on the lysate of Lp(a)-treated cells allowed to identify the highest intracellular accumulation of Lp(a) at 72 h treatment. Already at 24 h, however, important morphological alterations were detected upon Oil red and Nile red staining. A three-dimensional reconstruction obtained by two-photon scanning microscopy of the intracellular distribution of Nile red stained structures in treated cells shows preferential uptake of lipids in extra-nuclear regions. These data are useful to clarify the temporal and spacial aspects of intracellular accumulation of Lp(a) in RAW 264.7 cells and pose new bases for future studies on intracellular Lp(a) accumulation.
Authors and Affiliations
Maria Valentina Pasquetto, Alice Santonastaso, Alessandra Tomaselli, Patrizia Vaghi, Elton Hasani, Luca Tartara, Claudia Scotti
Keywords
Related Articles
- EP ID EP342021
- DOI 10.9734/BJMMR/2016/27433
- Views 61
- Downloads 0
Evolution and Exploration of Azadirachta indica in Dentistry: An Update
Despite great achievements globally in oral health of populations, dental caries and periodontal diseases are the most important oral health burdens particularly among under-privileged groups in developed and developing...
Cardiac Abnormalities in HIV Infected Children Presenting to a Tertiary Level Teaching Hospital at New Delhi
Aims: The aim of our study was to study the prevalence of various cardiac abnormalities in HIV positive children. Study Design: Cross sectional observational study. Place of Study: Antiretroviral treatment clinic of Mau...
Periodontal Health Status of Pregnant Women Attending Various Government Hospitals in Faridabad City, Haryana, India
Aims: The aim of the present study was to find out the periodontal health status among pregnant women attending various government hospitals in Faridabad city. Methodology: This was a descriptive cross sectional single s...
A Case of Chronic Aortic Dissection with Medial Calcification
Aims: Cystic medial necrosis is the most common pathological change of dissection. Medial calcification is mainly recognized in peripheral arteries and arterioles, but it can occur in the aorta. We, herein, report a case...
Cardiac Angiosarcoma of the Right Atrium with Cardiac Tamponade
Aims: We report a case of cardiac sarcoma, which showed cardiac tamponade as the first clinical symptom and sign. Accumulation of such cases could lead to better treatment for patients with cardiac angiosarcoma. Present...