Lipoprotein (a) Particles Characterization by Dynamic Light Scattering
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 16, Issue 10
Abstract
Lp(a) is a novel cardiovascular risk factor resembling an LDL particle. It includes a copy of apolipoprotein (a) [apo(a)], whose molecular weight is dependent on the number of genetically encoded kringle IV type 2 (KIV-2) repeats and inversely related with Lp(a) plasma concentration and risk. The reason for this inverse relationship is unclear and, particularly, there are no data regarding the size of Lp(a) particles carrying apo(a) with different molecular weights. The aim of the present work was to explore if a relationship existed between apo(a) molecular weight and particles size in Lp(a) samples carrying 20, 25 and 28 KIV-2 repeats (K20, K25 and K28, respectively). Dynamic Light Scattering (DLS) measurements were performed on affinity-purified Lp(a). A preliminary finding was that particles were typically distributed into three different size groups instead of the single one expected. No difference in average particle size between Lp(a) carrying different apo(a) isoforms was found. However, the percentage of medium-sized particles in each sample was found to be inversely related to the number of KIV-2 repeats (R2=0.99), with a clear predominance in K20 (58.53%). These data deserve further investigations, as they might be potentially relevant to explain the pathogenic role of low molecular weight Lp(a) isoforms.
Authors and Affiliations
Valentina Pasquetto, Alice Santonastaso, Stefania Grandi, Giuseppe Derosa, Angela D'Angelo, Claudia Scotti
Keywords
Related Articles
- EP ID EP341167
- DOI 10.9734/BJMMR/2016/27076
- Views 75
- Downloads 0
The Effect of Two Types of Statins (Rosuvastatin and Atorvastatin) on the Fertility of Male and Female Mice
Background: Studies showed controversial results regarding the effect of statins on fertility parameters. Several studies present evidence that statins have deleterious effects, others failed to prove these effects. Aim:...
Efficacy of Long-term Effect and Repeat Intraarticular Botulinum toxin in Patients with Painful Total Joint Arthroplasty: A Retrospective Study
Objective: Based on recent success of intra-articular (IA) Botulinum neurotoxin type A (BoNT/A; OnabotulinumtoxinA) in patients with osteoarthritis, we examined if repeat IA-BoNT/A is an effective antinociceptive in pat...
Myeloma Presenting as Paraparesis: A Case Report
Aim: To highlight the need to consider multiple myeloma as a probable diagnosis while evaluating a patient presenting with paraparesis and backache. Presentation of Case: A 55 year old woman presented to a hospital in Ca...
Computational DIO2 rSNP Analysis, Transcriptional Factor Binding Sites and Disease
Purpose: The DIO2 gene transcribes the deiodinase type 2 enzyme that changes the thyroid prohormone, thyroxine (T4), to the biologically active triiodothyronine (T3) hormone. T3 plays a vital part in the regulation of en...
Prevalence and Risk Factors of Lactation Mastitis in Three Hospitals in Cameroon: A Cross-Sectional Study
Background: Lactation mastitis is a painful, debilitating condition that if not well managed, can mislead women into prematurely stopping breastfeeding and by so doing deprive the child of optimal feeding. The World Heal...