LIQUID CHROMATOGRAPHY METHOD DEVELOPMENT AND VALIDATION FOR ANALYSIS OF CLONIDINE IN PHARMACEUTICAL DOSAGE
Journal Title: Journal of Atoms and Molecules - Year 2012, Vol 2, Issue 1
Abstract
A new simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid chromatography assay has been developed and validated for the estimation of CLONIDINE in tablet formulation. The separation was achieved by using C-18 column (250x4.6mm, 5μm in particle size) at ambient temperature coupled with a guard column of silica in mobile phase Methanol : Acetonitrile : water with the pH value adjusted to 5.8 .The flow rate of was 1.5ml/min and the drug was detected using UV detector at the wavelength of 280nm and the run time was 6min. The retention time was within 2.6minutes. The percentage of RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per ICH guidelines. The proposed method was found to be accurate, repeatability and consistent. It was successfully applied for the analysis of the drug in marketed formulation and could be effectively used for the routine analysis of formulation containing the drug without any alteration in the chromatography conditions.
Authors and Affiliations
Narendra Devanaboyina, B Charan Kumar, B Vijay, M. A Bhanu, V Gayathri
Keywords
Related Articles
- EP ID EP367400
- DOI -
- Views 133
- Downloads 0
RP – HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ANALYSIS OF TENOFOVUIR IN PHARMACETICAL DOSAGE FORMS
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of TENOFOVUIR in tablet dosage form. Isocratic elution at a flow rate of 1ml min-1 was employed on a symmetry C...
EFFECT OF BLANCHING ON NUTRIENT AND ANTI-NUTRIENT LEVEL OF LEAVES OF SOME VARIETIES OF CASSAVA (Manihot esculenta C.)
Random samples of leaves of three widely cultivated cassava varieties in Nigeria; TMS 30555, NR 8083 and TMS 30211 were collected from the Akwa Ibom State Agricultural Development Program (AKADEP) farm in Abak Local Gove...
A HERBAL PLANT CASSIA ANGUSTIFOLIA.
Senna is an Arabian name and the herb was first brought into use by the Arabian physicians Serapion and Mesue. Traditionally Senna was used in love sachets. Plants growing at different localities of India have also been...
PHYTO CHEMICAL SCREENING AND ISOLATION OF COUMARIN COMPOUNDS FROM MEDICINAL PLANT VACHA BARK
Vacha plant is medicinally useful plant. In order our phytochemical screening we identified a coumarins in Vacha plant. We are extracted and isolated coumarin and identified a compound in the Vacha bark.