Local and Systemic Imbalance of CD4+ T Cells in Granulomatous Colitis: A Comparative study
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 1
Abstract
Background and study aims: T helper cells and the various cytokines secreted by them play a crucial role in the pathogenesis of Intestinal tuberculosis (ITB) and Crohn’s disease (CD). We aimto study the levels of five cytokines (IL-4, IL-6, IL-17, IFN-ϒ & TGF-β1 ) in serum of patients with CD & ITB which indirectly reflects the levels of CD4+ T cells. Methodology: An observational, cross-sectional study done in a tertiary care hospital for 2 years. Patients with features of CD or ITB were included, with 12 cases of CD&13 cases of ITBand 20 controls. Colonoscopic biopsies were taken from the ulcerated and normal mucosa along with the patient’s peripheral blood. The tissues were processed for histopathological examination. Serum levels of five cytokines (IL-4, IL-6, IL-17, IFNϒ & TGF-β1) were estimated by ELISA along with flow cytometric estimation of T helper cell populations in the peripheral blood. Results: We found a significant imbalance among T helper cells in CD and ITB. This correlated with the levels of cytokines in the peripheral blood, of which mean IL-6 and IL-17 levels showed a statistically significant increase in CD. In addition, IFN- γ was also significantly increased, probably as a result of the increase in Th1 and Th17 cells. There was no change in IL4 levels. Conclusion: Cytokines have an important role in the pathogenesis of granulomatous inflammatory bowel diseases. The role of cytokines in their pathogenesis might be helpful for future therapy based on anti-cytokine antibodies
Authors and Affiliations
Dr Keya Basu, DrVinay Anand, DrDipan Das, DrRajib Sarkar, DrSuchorita Sarkar, DrKshaunish Das
Keywords
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- EP ID EP434304
- DOI 10.9790/0853-1801077983.
- Views 45
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