Local Xenotransplantation of Bone Marrow Derived Mast Cells (BMMCs) Improves Functional Recovery of Transected Sciatic Nerve in Cat: A Novel Approach in Cell Therapy
Journal Title: Bulletin of Emergency And Trauma - Year 2018, Vol 6, Issue 2
Abstract
Objective: To determine the effects of bone marrow derived mast cells (BMMCs) on functional recovery of transected sciatic nerve in animal model of cat. Method: A 20-mm sciatic nerve defect was bridged using a silicone nerve guide filled with BMMCs in BMMC group. In Sham-surgery group (SHAM), the sciatic nerve was only exposed and manipulated. In control group (SILOCONE) the gap was repaired with a silicone nerve guide and both ends were sealed using sterile Vaseline to avoid leakage and the nerve guide was filled with 100 μL of phosphate-buffered saline alone. In cell treated group ([SILOCONE/BMMC) the nerve guide was filled with 100 μL BMMCs (2× 106 cells/100 μL). The regenerated nerve fibers were studied, biomechanically, histologically and immunohiscochemically 6 months later. Results: Biomechanical studies confirmed faster recovery of regenerated axons in BMMCs transplanted animals compared to control group (p<0.05). Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in BMMCs transplanted animals than in control group (p<0.05). In immunohistochemistry, location of reactions to S-100 in BMMCs transplanted animals was clearly more positive than that in control group. Conclusion: BMMCs xenotransplantation could be considered as a readily accessible source of cells that could improve recovery of transected sciatic nerve.
Authors and Affiliations
Rahim Mohammadi, Dana Anousheh, Mohammad-Hazhir Alaei, Amin Nikpasand, Hawdam Rostami, Rasoul Shahrooz
Keywords
Related Articles
- EP ID EP314008
- DOI 10.29252/beat-060204
- Views 49
- Downloads 0
Delayed Cardiac Rupture Induced by Traumatic Myocardial Infarction: Consequence of a 45-Magnum Blast Injury; A Comprehensive Case Review
A penetrating chest trauma, a myocardial contusion or a myocardial infarction can lead to a cardiac rupture, which is linked to an extreme high death rate. Only few cases with delayed perforation of the myocardium have b...
Epidemiologic Aspects of Overall Injuries in Hamadan Province; A Six-Year Registry-Based Analysis
Objective: To investigate the epidemiological aspects of all-cause injuries in Hamadan province during a 6-year period. Methods: We conducted a cross-sectional study on injured subjects admitted to private or governmenta...
The Predictive Value of Repeated Abdominal Ultrasonography in Patients with Multiple Trauma and Decreased Level of Consciousness: The Experience of a Resource-Limited Centre
Objective: To determine the predictive value of repeated abdominal ultrasonography in patients with multiple trauma and decreased level of consciousness (LOC). Methods: This prospective cross-sectional study was conducte...
Heart Rate Beat to Beat Variability of Trauma Patient in Neurogenic Shock State: Time to Introduce New Symptoms
The autonomic nervous system (ANS) is consisted of several centers in the central nervous system (CNS) and the connections with the end organs through plexus and peripheral nerves which controls the hemodynamics [1]. It...
Enhanced Recovery after Emergency Surgery: A Systematic Review
Objective: To evaluate the current scientific evidence for the applicability, safety and effectiveness of pathways of enhanced recovery after emergency surgery (ERAS). Methods: We undertook a search using PubMed and Coch...