Local Xenotransplantation of Bone Marrow Derived Mast Cells (BMMCs) Improves Functional Recovery of Transected Sciatic Nerve in Cat: A Novel Approach in Cell Therapy

Journal Title: Bulletin of Emergency And Trauma - Year 2018, Vol 6, Issue 2

Abstract

Objective: To determine the effects of bone marrow derived mast cells (BMMCs) on functional recovery of transected sciatic nerve in animal model of cat. Method: A 20-mm sciatic nerve defect was bridged using a silicone nerve guide filled with BMMCs in BMMC group. In Sham-surgery group (SHAM), the sciatic nerve was only exposed and manipulated. In control group (SILOCONE) the gap was repaired with a silicone nerve guide and both ends were sealed using sterile Vaseline to avoid leakage and the nerve guide was filled with 100 μL of phosphate-buffered saline alone. In cell treated group ([SILOCONE/BMMC) the nerve guide was filled with 100 μL BMMCs (2× 106 cells/100 μL). The regenerated nerve fibers were studied, biomechanically, histologically and immunohiscochemically 6 months later. Results: Biomechanical studies confirmed faster recovery of regenerated axons in BMMCs transplanted animals compared to control group (p<0.05). Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in BMMCs transplanted animals than in control group (p<0.05). In immunohistochemistry, location of reactions to S-100 in BMMCs transplanted animals was clearly more positive than that in control group. Conclusion: BMMCs xenotransplantation could be considered as a readily accessible source of cells that could improve recovery of transected sciatic nerve.

Authors and Affiliations

Rahim Mohammadi, Dana Anousheh, Mohammad-Hazhir Alaei, Amin Nikpasand, Hawdam Rostami, Rasoul Shahrooz

Keywords

Related Articles

Transradial, Transscaphoid, Transcapitate, Perilunate Dislocation; A Case Report and Approach to the Patient

Carpal injuries are uncommon and are mainly seen in the younger age group. The incidence is unclear as many of the cases go undiagnosed. A 22-year-old male patient coming to the hospital after road traffic accidentwith h...

Psycho-social and Mental Variables and Post-Traumatic Stress Disorder in Traffic Accident Survivors in Northern Iran

Objective: To assess the psycho-social and mental variables associated with post-traumatic stress disorder (PTSD) in a series of Iranian patients. Methods: A total of 528 eligible accident survivors in pre-sampling of a...

Mishra’s Sign of Blunt Traumatic Pancreatic Injury’: An Intra-Operative Telltale Sign Indicating Potential Blunt Traumatic Pancreatic Injury

Dear Editor Missed traumatic pancreatic injuries especially injury to the main pancreatic duct (MPD) is a small but significant cause for morbidity and mortality [1, 2]. During exploratory laparotomy, traumatic pancreati...

Risk Factors of Road Traffic Accidents Associated Mortality in Northern Iran; A Single Center Experience Utilizing Oaxaca Blinder Decomposition

Objectives: To investigate the differences in death after receiving emergency services in traffic accidents between urban and rural regions, and decompose factors of the gap in Langerood, Northern Iran. Methods: This cro...

Gangrenous Appendicitis: A Rare Presentation of Pericecal Hernia; Case Report and Review of the Literature

Intra-abdominal Hernias are rare conditions usually secondary to congenital defects, with pericecal hernias accounting for a minority of them. They can be difficult to diagnose due to non-specific presentations but may l...

Download PDF file
  • EP ID EP314008
  • DOI 10.29252/beat-060204
  • Views 55
  • Downloads 0

How To Cite

Rahim Mohammadi, Dana Anousheh, Mohammad-Hazhir Alaei, Amin Nikpasand, Hawdam Rostami, Rasoul Shahrooz (2018). Local Xenotransplantation of Bone Marrow Derived Mast Cells (BMMCs) Improves Functional Recovery of Transected Sciatic Nerve in Cat: A Novel Approach in Cell Therapy. Bulletin of Emergency And Trauma, 6(2), 108-114. https://europub.co.uk/articles/-A-314008