Long Term Consumption of Coconut Oil Diet Increased Anxiety Related Behaviour in CD1 Mice
Journal Title: Journal of Complementary and Alternative Medical Research - Year 2017, Vol 2, Issue 1
Abstract
The effect of prolonged consumption of virgin coconut oil (VCO) diet on anxiety was assessed in CD1 mice using the Open field (OF), Elevated plus maze (EPM) and Light/dark box (LD box) tests. Thirty male CD1 mice (28.4 - 32.5 g bw) divided into 3 groups (n=10 each) were given normal rodent chow (control), 5% (w/w) and 20% (w/w) VCO diet respectively and water ad libitum for 23 days. The OF test showed increased grooming (p< 0.05) in both 5% and 20% VCO groups versus control, and higher Freeze frequency in both VCO diet groups versus control, with the 20% diet group being higher than that for 5% diet group (p<0.05). Stretch attend postures (SAP) were similarly higher in the 5% and 20% diet groups (P<0.05) versus control, and higher (P<0.05) in the 20% VCO diet versus 5% VCO diet group. In the EPM test, the open arms duration was lower (p< 0.05) in the 20% VCO diet group. SAPs in the EPM test were higher in both 5% and 20% VCO diet groups (p< 0.05.), with that for the 20% VCO diet group being higher than that for the 5% VCO group (p< 0.05). In the LD box test, SAP was significantly higher in 20% VCO diet group versus control (p<0.05). All behaviours scored in the three tests indicate increased anxiety in the VCO diet groups. Thus long-term consumption of virgin coconut oil diet increased anxiety-related behaviour in the mice, with the 20% diet causing a greater effect.
Authors and Affiliations
S. A. Bisong, U. A. Okon, J. A. O. Chukwu, O. A. Sanya, M. A. Akinnuga, G. N. Unirere
Keywords
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- EP ID EP337088
- DOI 10.9734/JOCAMR/2017/30315
- Views 105
- Downloads 0
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