Lutein and β-Carotene in Selected Asian Leafy Vegetables
Journal Title: Journal of Food Chemistry and Nanotechnology - Year 2017, Vol 3, Issue 3
Abstract
Carotenoids, such as lutein and β-carotene are generally recognized as antioxidants. However, only β-carotene has the pro-vitamin A activity. This study determined lutein and β-carotene in Asian leafy vegetables using reversed-phase high pressure liquid chromatography (HPLC). The selected samples were Chinese mustard (Brassica juncea var. rugose), water spinach (Ipomoea aquatic), Chinese spinach (Amaranthus tricolor), coriander (Coriandrum sativum), Chinese flowering cabbage (Brassica parachinensis), Chinese broccoli (Brassica oleracea alboglabra) and Chinese white cabbage (Brassica chinensis). Carotenoids were extracted from samples into 5% (w/v) calcium carbonate and acetone. The extracts were then concentrated at 4 °C and filtered prior to HPLC analysis. The recoveries for β-carotene and lutein were 132% and 100%, respectively. The coefficient of variation for inter-variability study was < 3.8% and for intra-variability study < 11%. The range of β-carotene in the selected vegetables was 1974-6604 μg/100 g (wet weight basis), or 329-1101 μg/100 g retinol equivalent (RE). Measured lutein contents were between 694- 5919 μg/100 g. Chinese broccoli had the highest pro-vitamin A activity (1101 μg RE per 100 g). The β-carotene data reported here was useful for estimating the pro-vitamin A intake from consuming the Asian leafy vegetables.
Authors and Affiliations
Maria V. Chandra-Hioe, Hassan H. Rahman, Jayashree Arcot
Keywords
Related Articles
- EP ID EP223395
- DOI 10.17756/jfcn.2017-043
- Views 118
- Downloads 0
Harmful Effects on Oryzaephilus surinamensis (L.) and Tribolium castaneum by Food Grade Antioxidants and their Formulations in Peanut Kernel
The aim of this study was to investigate effect of 2(3)-tert-butyl-4 hydroxyanisole (BHA) and 2,6-di(tert-butyl)-p-cresol (BHT) and their formulations on mortality, body weight and total protein content of Oryzaephilus s...
Classification of Bacillus cereus and Bacillus thuringiensis by Metabolomic Profile Differentiation Using Ultra- Performance Liquid Chromatography-Tandem Mass Spectrometry
Bacillus species are aerobic spore-forming bacteria that are known pose a significant risk to human health. Even though numerous methods for classifying Bacillus cereus and Bacillus thuringiensis have been reported, it r...
Chlorogenic Acid Profiles and Antioxidant Potentials of 17 Sweet Potato Varieties Cultivated in Korea: Impact of Extraction Condition and Classification by Hierarchical Clustering Analysis
A high resolution LC-ESI-QTOF-MS, LC-MS/MS, and HPLC-DAD analysis were conducted to isolate, identify and quantify the chlorogenic acids in 17 sweet potato varieties cultivated in Korea. The impacts of extracting solvent...
A Review on Microextraction Techniques for Selected Triazole Fungicides Determination in Water and Food Samples
Triazole fungicides are systemic fungicides applied to protect crops from fungus infections. Its use must be controlled due to the potential harmful effects in environment and human health. Before determination with a ch...
Effect of Herbal Antimicrobials on Bacterial Strains of Foods of Vegetable and Animal Origin
At the face of emergence of multiple antibiotic resistant strains, herbal antimicrobials are looked as the future drugs for therapeutics, and also as food preservative. This study was undertaken to understand the antimic...