Magnesium on Blockage of Nuclear Factor-B Activation at Ventilator-Induced Lung Injury: Random Trial
Journal Title: Enliven: Journal of Anesthesiology and Critical Care Medicine - Year 2015, Vol 2, Issue 7
Abstract
Rationale The mechanical ventilation may aggravate the severe acute lung injury and trigger a systemic inflammatory response. The pathophysiology evolves Biotrauma, meaning that the over distension and the cyclical opening/collapse of structures on the lung during ventilation with high PIP associated with low PEEP, results in excessive activation of nuclear factor-{kappa}Beta (NF-κB), a regulator of the inflammatory response, that exacerbates the local response and becomes it systemic. The magnesium, a modulator of signaling pathways of the inflammatory cascade, deserves investigation in inhibiting the NF-κB activation. Objectives Investigate experimentally the effect of hypermagnesemia to inhibit the activation and migration of NF-κB from cytoplasm to nucleus in cells of lungs injured by mechanical ventilation. Methods Two experimental groups of 15 Sprague-Dawley rats each, one receiving 1.25 mL/kg/h of a 10% MgSO4 solution, other receiving equal volume of saline, both under mechanical ventilation with VT of 40 mL/kg, PEEP of 3 cmH2O, PIP of 35 cm H2O, RR of 40 cycles per minute. Lungs were excised after 50 minutes of experimental interventions and prepared for further immunohistochemical analysis. Measurements and Main Results ANOVA shown no differences when compared the NF-κB activation values in the cytoplasm (712.44 ± 253.86) and nucleus (974.27 ± 344.59) of cells in MgSO4 group with the respective values in cytoplasm (653.99 ± 272.98) and nucleus (952.29 ± 346.68) of cells in Saline group (F[1, 28] = 0.90, p > .05). Conclusions There was no inhibitory effect of magnesium on the activation of NF-kB nor on its expression through migration to the nucleus.
Authors and Affiliations
Grigolli Cesar Regina, De Carvalho Werther Brunow
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