Magnetic Solid Lipid Nanoparticles Loading 5-Fluorouracil to Colon Cancer Treatment
Journal Title: Scholars Academic Journal of Pharmacy - Year 2018, Vol 7, Issue 4
Abstract
Abstract: Magnetic solid lipid nanoparticles (MSLNs) made of iron oxide cores embedded within a glyceryltrimyristate solid matrix have demonstrated hem compatibility, appropriate magnetic response and adequate heating characteristics to be used in hyperthermia. We now tested the association of MSLNs to 5-fluorouracil (5-FU), a drug of chose in colon cancer treatment, to determine the ant proliferative effect modulation. We used HCT15 resistant and T-84colon cancer cells. The non-magnetic solid lipid nanoparticles (SLNs) were included as a control. In addition, we analyze the effect of both MSLNs and SLN in relation to the P-glycoprotein activity, a resistance mechanism implicated in colon cancer chemotherapy failure. Our results showed that both MSLNP and SLNP did not induce cell toxicity and that both NPs loading 5-FU (SLNP-5-FU and MSLNP-5-FU) did not modulate free 5-FU activity against T-84 and HCT15 cells. In addition, fluorescent microscopy analysis showed that none of the NPs was able to decrease rhodamine 123 into tumor cells indicating absence of activity in relation to P-glycoprotein pump. In conclusion, MSLNs could be used as an anticancer therapeutic system not only by their demonstrated hyperthermic properties but as a 5-FU transporter. Future studies will be necessary to know the applicability of this dual system (hyperthermia/drug) against colon cancer.
Authors and Affiliations
Laura Cabeza, María M. de Escalona, Gloria Perazzoli, Eva Sáez-Fernández, José L. Arias
Keywords
Related Articles
- EP ID EP480170
- DOI -
- Views 85
- Downloads 0
Stability Indicating Method Development and Validation for Simultaneous Estimation of Mefloquine and Artesunate in Tablet Dosage Form
The main objective of this study was to develop a simple, efficient, specific, precise and accurate Stability indicating Reverse Phase High Performance Liquid Chromatographic method for estimation of Mefloquine and Artes...
Evaluation of Analgesic and Anti Inflammatory Activities of Ethanolic Extracts of Medicinal Plants Baccaurearamiflora andMicrocospaniculata
Baccaurearamiflora(family: Euphorbiaceae) is native to Southeast Asia region and is found distributed in the sub-Himalayan tract, mainly from Nepal to Sikkim, Darjeeling hills, Arunachal Pradesh, Tripura, Assam, Bhutan,...
A facile method of Synthesizing Ayurvedic medicine: Lauha bhasma (iron oxide nanoparticles) & its Characterization
Iron oxide nano particles can be synthesized by various physical and chemical methods which are toxic and harmful for the environment. Ayurveda is a holistic and divine science; a modern method of synthesizing ayurvedic...
Effects of Ethanolic Extract of Bauhinia Variegata Leaves Alone and In Combination with Metoclopramide on Induced Model of Acute Colitis in Rabbits
Abstract: The exact reasons of UC are still unidentified; however, numerous factors have been implicated in the pathophysiology of the disease. Inflammatory mediators such as cytokines, reactive oxygen species, also 5-HT...
Studies on the Amino Acid Composition and Antioxidant Activities of Collagen Polypeptides from HolothurianobilisSelenka
Abstract:Studies on amino acid composition and antioxidant activities of collagen Polypeptides from the HolothurianobilisSelenka were carried out in this paper. Results revealed that collagen polypeptides mainly contains...