Magnetic Solid Lipid Nanoparticles Loading 5-Fluorouracil to Colon Cancer Treatment
Journal Title: Scholars Academic Journal of Pharmacy - Year 2018, Vol 7, Issue 4
Abstract
Abstract: Magnetic solid lipid nanoparticles (MSLNs) made of iron oxide cores embedded within a glyceryltrimyristate solid matrix have demonstrated hem compatibility, appropriate magnetic response and adequate heating characteristics to be used in hyperthermia. We now tested the association of MSLNs to 5-fluorouracil (5-FU), a drug of chose in colon cancer treatment, to determine the ant proliferative effect modulation. We used HCT15 resistant and T-84colon cancer cells. The non-magnetic solid lipid nanoparticles (SLNs) were included as a control. In addition, we analyze the effect of both MSLNs and SLN in relation to the P-glycoprotein activity, a resistance mechanism implicated in colon cancer chemotherapy failure. Our results showed that both MSLNP and SLNP did not induce cell toxicity and that both NPs loading 5-FU (SLNP-5-FU and MSLNP-5-FU) did not modulate free 5-FU activity against T-84 and HCT15 cells. In addition, fluorescent microscopy analysis showed that none of the NPs was able to decrease rhodamine 123 into tumor cells indicating absence of activity in relation to P-glycoprotein pump. In conclusion, MSLNs could be used as an anticancer therapeutic system not only by their demonstrated hyperthermic properties but as a 5-FU transporter. Future studies will be necessary to know the applicability of this dual system (hyperthermia/drug) against colon cancer.
Authors and Affiliations
Laura Cabeza, María M. de Escalona, Gloria Perazzoli, Eva Sáez-Fernández, José L. Arias
Keywords
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