Mahkota Dewa Mesocarp Infusion Improved Testicular Damage and Sperm Count In Diabetic Rat
Journal Title: SAINS MEDIKA (Journal of Medicine and Health) - Year 2012, Vol 4, Issue 2
Abstract
Background: Global prevalence of Diabetes Mellitus (DM) is predicted to be increasing with worldwide population increase, longer life expectancy, urbanization, change in lifestyle and higher incidence of obesity. Diabetes Mellitus which is characterized by increasing level of blood glucose can induce increasing activity of ROS (Reactive Oxygen Species) which can further lead to abnormality in male reproductive system, especially testis. Mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.=PM) contain flavonoid, alkaloid and saponin which can prevent further negative effect on ROS increase in male reproductive system. The aim of this study was to know effect of PM mesocarp infusion on testicular damage and sperm count in alloxan induced diabetic rat. Design and Method: The design of this study was post test only with control group design. Rats in this study were divided into 4 groups (6 rats each group): control group, diabetic group, diabetic group treated with PM mesocarp infusion (9% and 13.5%). Diabetes was induced by alloxan 50 mg/BW single dose injection intramuscularly. PM mesocarp’s infusion was given in 4 weeks. Testicular damage was observed in hematoxylin-eosin stain using Johnsen criteria for spermatogenesis level and tubulus seminiferous’ diameter. Sperm count was performed on the semen taken from vas defferens using Giemsa stain. Result: PM mesocarp infusion group has higher spermatogenic level (p < 0,05), higher tubulus seminiferus diameter and sperm count (p < 0,05) than diabetic group. Conclusion: Mahkota dewa mesocarp infusion improved testicular damage and sperm count in alloxan-induced diabetic rat (Sains Medika, 4(2):115-123).
Authors and Affiliations
Evy Sulistyoningrum, Setiawati . , Herlina Nindyastuti, Adrian Nugraha Putra
Keywords
Related Articles
- EP ID EP234505
- DOI -
- Views 101
- Downloads 0
Antioxidant Activities and Antioxidant Cream Formulation of Corn Silk (Zea Mays L) Extract
Introduction : One of the free radicals that can damage facial skin is coming from UV radiation of the sun. Free radicals are the main factors that can accelerate the early aging process. The administration of antioxidan...
The Definitive Diagnosis of Tuberculous Meningitis (TBM) di RSUP dr. Kariadi Semarang Descriptive Study in Adult Patients using IS6110-based PCR Amplification for Detecting Mycobacterium tuberculosis
Tuberculous meningitis (TBM) is the most common cause of central nervous system infection and has very high morbidity and mortality. The definitive diagnosis of TBM in RSUP dr Kariadi Semarang has been insufficient. This...
Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice
Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on level...
Efficacy Assessment of Lemon Peel Aromatherpy Againts Airborne Bacteria Experimental Study in ICU Room of Sultan Agung Islamic Hospital Semarang
Background: Healthcare associated infections (HAI) are infections acquired in health care centers such as hospital, clinic or other health care sites due to microorganisms. Research conducted by WHO showed 3- 10% of all...
The Effect of Alium Satium Extract on the Glomerular Diameter of STZ -induced Sprague dawley Rats
Hyperglycemia exert toxic effect in kidney.The aim of this study was to investigate the short term effect of ethanolic extract of garlic in preventing nephropathy following streptozotocin (STZ) induced rats. Twenty male...