Manipulation of histochemical procedure for detection of uridine Diphospho - galactose 4 – Epimerase enzyme in the tissue of rat
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2018, Vol 17, Issue 2
Abstract
There are many techniques used for the detection of the activity of the cells. One of these measures is detection of the presence and activity of cell’s enzymes using histo-chemical method. One of these enzymes is Uridine Diphospho-Galatose 4-Epimerase. It’s activity could be estimated using simultaneously coupling method of Diculescu et al method. However this method has technical problem. This problem is the incubation medium is turbid which leads to get tissue slide with a lot of artifact and debris giving false positive result. To overcome this problem, many solvents had been used to have clean incubation medium, without inhibiting the activity of the enzyme, and to get clean and clear tissue slide could be obtained. The abstract should summarize the content of the paper. Try to keep the abstract below 200 words. Do not make references nor display equations in the abstract. The journal will be printed from the same-sized copy prepared by you. Your manuscript should be printed on A4 paper (21.0 cm x 29.7 cm). It is imperative that the margins and style described below be adhered to carefully. This will enable us to keep uniformity in the final printed copies of the Journal. Please keep in mind that the manuscript you prepare will be photographed and printed as it is received. Readability of copy is of paramount importance.
Authors and Affiliations
Shifaa Jameel Ibrahim
Keywords
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- EP ID EP579239
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