Manual Versus Automated Volume Reduction of Cord Blood
Journal Title: European Journal of Therapeutics - Year 2022, Vol 28, Issue 2
Abstract
Objectives: All cord blood banks all over the world follow a common procedure, concentrating progenitor cells by volume reduction, with the main purpose of optimizing the use of storage space. The main objective of this study was to compare CD34 and total nucleated cell recovery rates and red blood cell depletion efficiencies following cord blood processing using automated Sepax or manual CellEffic cord blood processing systems. Methods: Nine cord blood units with high volumes were divided into 2 equal fractions and processed with CellEffic cord blood and Sepax. Total nucleated cell, mononuclear cells, CD34+, red blood cell and total nucleated cell viability, and clonogenic assays were performed, and recovery rates were calculated on pre- and post-process cord blood units and after freeze/thaw process. In the comparison group, post-thaw differential cell counting was also performed. Results: Our results showed that post-process total nucleated cell viability with CellEffic cord blood was slightly higher than Sepax, whereas Sepax post-process total nucleated cell/ mononuclear cell values were superior to CellEffic cord blood. Postthaw red blood cell depletion was better for CellEffic cord blood. Post-thaw Sepax colony-forming unit counts were higher than CellEffic cord blood. In addition, CD45+CD71+ cells were lower, whereas CD45+CD34+CD38− cells were higher for the CellEffic cord blood system. Conclusion: Despite the fact that there is a need for well-trained personnel for processing cord blood units with CellEffic cord blood, it may be an attractive alternative to Sepax system for cord blood processing, particularly for cord blood units with low volumes, at banks with low budget where the cord blood turnover rates are relatively low.
Authors and Affiliations
Pınar Yurdakul Mesutoğlu, Emine Begüm Gencer Öncül, Hasan Yalım Akın, Meral Beksaç
Keywords
Related Articles
- EP ID EP720510
- DOI -
- Views 34
- Downloads 0
Scary Acute Left Main Coronary Artery Thrombus as an Initial Presentation of a Hereditary Thrombophilia: When to Go Out of Routine?
Patients with either hereditary or acquired thrombophilia can present with arterial and venous thrombotic complications. However, it is unclear to whom the thrombophilia panel should be assessed, particularly in patient...
Scoliosis After Liver Transplantation in Pediatric Patients
Objective: Little is known about the development of scoliosis after pediatric liver transplantation. In this study, we aimed to evaluate the frequency of scoliosis and its relationship with potential risk factors in chi...
New Age Borders Obtained from Spot Photoscreener by Using Multivariate Cluster Analysis
Objective: The aim of this study is to analyze all the variables obtained from photoscreener using hierarchical cluster analysis to create more homogeneous age groups for more reliable and consistent measurement by phot...
Evaluation of the Readability of Turkish Online Resources Related to Laryngeal Cancer
Objectives: The aim of this study was to investigate the readability of patient education materials in Turkish about laryngeal cancer. Methods: Patient education materials were determined by entering the term “gırtlak...
The Effect of Forced Exercise on Striatal and Serum Serotonin Levels in a Parkinson’s Mouse Model
Objective: The general treatment approach for Parkinson's disease (PD) is L-dopa administration. While L-dopa only relieves dopaminergic deficiency, it has no effect on the serotonergic system, which is thought to be im...