Markers characterizing corneal damage during aging of rat
Journal Title: JOURNAL OF ADVANCES IN CHEMISTRY - Year 2015, Vol 11, Issue 5
Abstract
Aging is a biological phenomenon that involves an increase of oxidative stress associated with gradual degradation of the structure and function of the cornea. Gender differences and subsequent deterioration of cornea is an interesting topic, especially yet few data are available concerning the impact of age, especially on the corneal. One hundred male and female Wistar albino rats ages 3, 6, 18, 24, and 30 months (n=10 equal for male and female) were used. At the time interval, cornea were investigated by light and transmission electron microscopy (TEM), immunohistochemistry of caspase 3 (casp3), glial fibrillar acidic protein(GFAP) and CD45 and flow cytometry of DNA, bcl-2-like protein 4 (BAX), transforming growth factor beta (TGF-β) and Cd45 (lymphocyte common antigen). Light and TEM investigation revealed apparent deterioration of atrophy of corneal epithelium with vesicular vacuolar degeneration, hyalinization of stromal collagen fibrils and swelling and degeneration of the endothelial lining the descemet's membrane. There was apparent loss of keratocytes within corneal stroma. Immunohistochemistry of casp 3 and CD45 were markedly increased manifesting cell damage. GFAP showed apparent reduction of innervation of corneal stroma and endothelium layer. Flow cytometry of DNA, Bax and TGF revealed increased apoptic cell death of cornea of 30M-old rats. We concluded that aging contributed to an apparent increase of cellular damage of different corneal region associated with alterations of cell markers.
Authors and Affiliations
Hassan I. H. El-Sayyad, Ahmed A. El-Mansi, Mona S Guida, Ezaldin AM Mohammed
Keywords
Related Articles
- EP ID EP652845
- DOI 10.24297/jac.v11i5.4471
- Views 155
- Downloads 0
Synthesis, Characterization and Antitumor Activity of Some New Oganotellurium Compounds Containing Azomethine Group, Part One
New tellurated schiff bases were synthesized by the reaction of the corresponding mercurated Schiff  bases compounds A1-A3 with tellurium tetrabromide in 1:1 mole ratio and  that  gave organyltellurium tribromides  A...
MAINTENANCE STRATEGY EVALUATION BASED ON AHP – TOPSIS
This paper presents an application of Analytical Hierarchy Process (AHP) is combined with Technique for Order Preference by Similarly to Ideal Solution (TOPSIS) model for selection of the best maintenance strategy for pu...
RAPID BLEEDING REGION DETECTION IN WIRELESS CAPSULE ENDOSCOPY VIDEOS
Wireless Capsule Endoscopy (WCC) is a medical imaging technique used to examine parts of the gastrointestinal tract. Computer aided detection is used to increase the speed of detection, better performance and reduce the...
4-(3,4-Dichlorophenyl)-6-(Furan-2-yl)Pyrimidine-2-Thiol as Building Block in Heterocyclic Synthesis
Pyrimidine-2-thiol (1) has been synthesized via one-pot, three component synthesis. Its behavior towards nitrogen nucleophiles like hydrazine hydrate, ethylamine, piperidine, and 2-aminobenzoic acid has been studied. A s...
POROUS BASED IMMUNOSENSOR FOR DETECTION OF LDL MOLECULES FROM BLOOD SERUM USING ARRAY OF CANTILEVER BEAM
In this work, the NiO thin film based array of Cantilever structure for the detection of low-density lipoprotein (LDL) is proposed. To detect the LDL molecules in the applied blood serum, the highly selective Anti-apolip...