Markers characterizing corneal damage during aging of rat
Journal Title: JOURNAL OF ADVANCES IN CHEMISTRY - Year 2015, Vol 11, Issue 5
Abstract
Aging is a biological phenomenon that involves an increase of oxidative stress associated with gradual degradation of the structure and function of the cornea. Gender differences and subsequent deterioration of cornea is an interesting topic, especially yet few data are available concerning the impact of age, especially on the corneal. One hundred male and female Wistar albino rats ages 3, 6, 18, 24, and 30 months (n=10 equal for male and female) were used. At the time interval, cornea were investigated by light and transmission electron microscopy (TEM), immunohistochemistry of caspase 3 (casp3), glial fibrillar acidic protein(GFAP) and CD45 and flow cytometry of DNA, bcl-2-like protein 4 (BAX), transforming growth factor beta (TGF-β) and Cd45 (lymphocyte common antigen). Light and TEM investigation revealed apparent deterioration of atrophy of corneal epithelium with vesicular vacuolar degeneration, hyalinization of stromal collagen fibrils and swelling and degeneration of the endothelial lining the descemet's membrane. There was apparent loss of keratocytes within corneal stroma. Immunohistochemistry of casp 3 and CD45 were markedly increased manifesting cell damage. GFAP showed apparent reduction of innervation of corneal stroma and endothelium layer. Flow cytometry of DNA, Bax and TGF revealed increased apoptic cell death of cornea of 30M-old rats. We concluded that aging contributed to an apparent increase of cellular damage of different corneal region associated with alterations of cell markers.
Authors and Affiliations
Hassan I. H. El-Sayyad, Ahmed A. El-Mansi, Mona S Guida, Ezaldin AM Mohammed
Keywords
Related Articles
- EP ID EP652845
- DOI 10.24297/jac.v11i5.4471
- Views 164
- Downloads 0
Corrosion Behaviour of Metals in Artificial Tear Solution
Human tear comes in contact with a number of instruments during operation in the eyes. This results in a variety of undesirable effects such as corrosion and malfunction. Corrosion behaviour of five metals, namely, m...
Voltammetric studies of some azo compounds derived from 4-methyl-6,7-dihydroxy coumarin in microemulsion and aqueous media
The cyclic voltammetric(CV) behavior of some azo compounds based on coumarin derivatives was investigated in microemulsion systems and in aqueous solutions. The obtained results indicated that these compounds undergo a...
ASSESSMENT OF SEASONAL PHYSICO-CHEMICAL PARAMETERS OF OGUTA LAKE, NIGERIA.
Seasonal variation of physico-chemical variables of Oguta Lake, Nigeria was studied. Results obtained using standard analytical methods indicated that in the rainy and dry seasons (July and August) and (October and Novem...
Equilibrium Study and Biological Activity of Cu(II) with Polyvinyl alcohol(PVA) and Some Amino acids and DNA
This study presents the acid-base equilibrium of polyvinyl alcohol (PVA). The stability constant values of the binary and ternary complexes formed in solution among polyvinyl alcohol, Cu (II), some amino acids and DNA we...
Processing of Spatio-Temporal Hybrid Search Algorithms in Heterogenous Environment Using Stochastic Annealing NN Search
In spatio-temporal database the mixed regions are present in a random manner. The existing work produces the result to create new research opportunities in the area of adaptive and hybrid SLS algorithms. This algorithm d...