Mechanism of the negative inotropic effect of naringin in mouse heart
Journal Title: Journal of Pharmacy & Pharmacognosy Research - Year 2014, Vol 2, Issue 5
Abstract
Context: Naringin (NRG) is the major flavonoid (flavanone glycoside) in grapefruit juice. Its biological activity has been only partially characterized and little is known about the mechanism of the negative inotropic action of this flavonoid. Aims: To evaluate the effects of NRG on the surface electrogram (ECG) and the force of contraction (FC) of mice hearts as well as on the sodium (INa), calcium (ICaL) and Na+ – Ca2+ exchange (INaCaX) currents of enzymatically isolated mouse ventricular cardiomyocytes. Methods: ECG and FC were recorded on mouse hearts perfused in a Langendorff column. Ventricular cardiomyocytes were enzimatically dissociated and ionic currents recorded with the patch-clamp technique. Results: NRG increased RR interval and shortened corrected QT only at high concentrations (30-100 µM). However, at a fixed heart rate, it decreased FC with an IC50 of 0.4 µM. NRG reduced INa with an IC50 of 0.07 µM but with a maximal inhibition of 60 %. NRG also depressed ICaL with an IC50 of 0.013 µM and increased its fast inactivation time constant. The effects on ICaL were not voltage-dependent. INaCaX was not affected by NRG. Conclusions: Our results indicate that NRG exerts a negative inotropic effect in mice hearts that could be explained by a decrease in INa and ICaL. These actions should be taken into account when considering this molecule either as a dietetic supplement or as a template to develop therapeutic agents for human diseases.
In vitro antimycobacterial activity of acetone extract of Glycyrrhiza glabra
Context: Glycyrrhiza glabra (licorice) has been used since ages as expectorant, antitussive and demulcent. G. glabra has been indicated in Ayurveda as an antimicrobial agent for the treatment of respiratory infections an...
Hepatoprotective and nephroprotective activities of Juniperus sabina L. aerial parts
Context: Previous studies indicated that Juniperus species exhibited promising hepatoprotective activity. Aims: To evaluate the hepatoprotective and nephroprotective activity of the total alcohol extract of the aerial p...
Historical development of epistemology and the study of pain: Place of neuromodulation of electroacupuncture in the experimental pain research
Despite the diffusion of acupuncture and its related techniques in Cuba and the World, its mechanism of action is still controversial, being considered by the most sceptics as placebo or some kind oriental myth, and it o...
Desmodium gangeticum root extract attenuates isoproterenol-induced cardiac hypertrophic growth in rats
Context: Desmodium gangeticum (L) DC (Fabaceae; DG), a medicinal plant that grows in tropical habitats, is widely used to treat various ailments including digestive and inflammatory disorders. Aims: To investigate the po...
Microbial and physicochemical assays of paracetamol in different brands of analgesic syrups sold in Sana’a City-Yemen
Context: Contamination of pharmaceuticals with microorganisms irrespective whether they are harmful or nonpathogenic can bring about changes in physicochemical characteristics of the drugs. Aims: To assay the microbial...