MED12 exon 2 mutations in uterine leiomyoma’s in women patients of Tehran Province of Iran
Journal Title: Scientific Journal of Biological Sciences - Year 2016, Vol 5, Issue 1
Abstract
Uterine leiomyomas or fibroids are benign smooth muscle tumors of myometrial origin; frequently found in reproductive age women. Uterine leiomyomas commonly cause significant symptoms such as anemia resulting from heavy irregular uterine bleeding, Pelvic discomfort, and bowel/bladder dysfunction from pressure. Fibroids have also been associated with infertility and recurrent abortion. Somatic mutations in MED12 exon 2 have recently been reported in uterine leiomyomas. The aim of our study was to see whether MED12 exon 2 mutations occur in uterine leiomyomas of women patients of Tehran Province of Iran. Mutations in exon 2 of the MED12 gene have been reported in 50% to 70% of uterine leiomyomas. To determine the frequency of MED12 mutations in various types of smooth muscle tumors as well as normal uterine myometrium adjacent to a leiomyoma, we selected a total of 23 cases for analysis of MED12 exon 2 mutations by polymerase chain reaction and Sanger sequencing. We found that 65℅ of classical uterine leiomyomas harbored mutations in exon 2 of the MED12 gene. Twelve of these mutations were located in codon 44 (c.130-132). In addition, one fibroids 0/43% displayed a missense mutation in codon 36 (c.107).We also observed two (0/86) exonic deletion type.
Authors and Affiliations
A. R. Ahmadi*| BioMedical Sciences Department, Alzahra University, Tehran, Iran., H. Porrahim| BioMedical Sciences Department, Alzahra University, Tehran, Iran., H. Moghadasi| BioMedical Sciences Department, Alzahra University, Tehran, Iran., N. Najafi| BioMedical Sciences Department, Alzahra University, Tehran, Iran., A. Rakhshan| Department of Pathology, Shohadaye Tajrish Hospital, Tehran, Iran.
Keywords
Related Articles
- EP ID EP1129
- DOI 10.14196/sjbs.v5i1.2043
- Views 481
- Downloads 27
The effects of shrubs salsola arbusculiformis Drob. on soil chemical and physical characteristics in North East rangelands of Iran
The purpose of this investigation was to study the effects of Salsola arbusculiformis on soil physico-chemical characteristics in rangelands of in Garmeh Located in northeast of Iran. After surveying the regions, the thr...
Time course for cellulase enzyme production by Aspergillus Flavus using different organic nitrogen sources
The time course for the production of cellulase enzyme of Aspergillus flavus was carried out at 35οC in a shaken incubator using modified Mandel and Weber’s medium containing in ( g/l) (NH4)2S04 1.4, KH2P04 2.0, Urea 0....
Haematological effects of aqueous extract of Vernonia amygdalina in wistar rats
The present study assessed the haematological effects of aqueous extract of (Vernonia amygdalina) in wistar rats. Fifteen (15) adult Wistar rats were used in this study. The animals were divided into three groups of five...
Genetic parameter estimation and model selection for hot carcass weight in matebele goat
Genetic parameter estimation for simple carcass traits has been confined to the improved goat breeds worldwide unlike in the unimproved breeds in developing countries where goats are numerous. Variance components for add...
Occurrence of deoxynivalenol in cereals and cereal based products: a short review
During recentdecades, the contamination of cereal grains with toxic metabolites of Fusariumspecies has been increasingly documented. Deoxynivalenol (DON) is one of themost common trichothecene toxins produced by F. grami...