MED12 exon 2 mutations in uterine leiomyoma’s in women patients of Tehran Province of Iran
Journal Title: Scientific Journal of Biological Sciences - Year 2016, Vol 5, Issue 1
Abstract
Uterine leiomyomas or fibroids are benign smooth muscle tumors of myometrial origin; frequently found in reproductive age women. Uterine leiomyomas commonly cause significant symptoms such as anemia resulting from heavy irregular uterine bleeding, Pelvic discomfort, and bowel/bladder dysfunction from pressure. Fibroids have also been associated with infertility and recurrent abortion. Somatic mutations in MED12 exon 2 have recently been reported in uterine leiomyomas. The aim of our study was to see whether MED12 exon 2 mutations occur in uterine leiomyomas of women patients of Tehran Province of Iran. Mutations in exon 2 of the MED12 gene have been reported in 50% to 70% of uterine leiomyomas. To determine the frequency of MED12 mutations in various types of smooth muscle tumors as well as normal uterine myometrium adjacent to a leiomyoma, we selected a total of 23 cases for analysis of MED12 exon 2 mutations by polymerase chain reaction and Sanger sequencing. We found that 65℅ of classical uterine leiomyomas harbored mutations in exon 2 of the MED12 gene. Twelve of these mutations were located in codon 44 (c.130-132). In addition, one fibroids 0/43% displayed a missense mutation in codon 36 (c.107).We also observed two (0/86) exonic deletion type.
Authors and Affiliations
A. R. Ahmadi*| BioMedical Sciences Department, Alzahra University, Tehran, Iran., H. Porrahim| BioMedical Sciences Department, Alzahra University, Tehran, Iran., H. Moghadasi| BioMedical Sciences Department, Alzahra University, Tehran, Iran., N. Najafi| BioMedical Sciences Department, Alzahra University, Tehran, Iran., A. Rakhshan| Department of Pathology, Shohadaye Tajrish Hospital, Tehran, Iran.
Keywords
Related Articles
- EP ID EP1129
- DOI 10.14196/sjbs.v5i1.2043
- Views 446
- Downloads 27
Effect of canola oil on blood lipid in ostriches (Struthio camelus)
In order to study the lipid profile of ostriches in relation to diet, 6 blue-neck male ostriches (Struthio camelus) were used and fed with 3% canola oil supplement on diets adlibitum consisting. In the morning, after abo...
Reproductive biology and histological characteristics of female little tunny Euthynnus alletteratus (Rafinesque, 1810) caught on continental shelf of Côte d’Ivoire
Some females little tunny (n=395) caught in gillnets and measuring in size from 35 to 76 cm FL (centimetre Fork Length) were sampled from January to December 2004 to study some biological features (length-weight relation...
Effects of aqueous crude leaf extract of senecio biafrae on the histology of the frontal cortex, kidney, liver and testis of male sprague dawley rats
Whenever any plant and/or herb is ingested, the body system interacts with it in an attempt to get rid of any harmful toxins such may contain, especially if the body cannot convert the foreign substance into useful compo...
Fertility and hatchability characterization of three strains of egg type chickens
The fertility and hatchability characteristics of three popular strainsof egg type chickens in Nigeria, namely chickens (ISABROWN), Alpha (Improved native) and the local strain (Pure native) chickenswere studied. They we...
Sero-prevalence of infectious bronchitis antibodies in local chickens in live bird markets in Sokoto State, Nigeria
A total of 161 apparently healthy male and female, grower and adult local chickens were sampled from Live bird Markets (LMB) in four Agricultural zones of the State; Gwadabawa, Tambuwal, Isa and Sokoto. They were screene...