Media Optimization For Lovastatin Production by Statistical Approach Using Aspergillus terreus by Submerged Fermentation
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 2
Abstract
Lovastatin is a secondary metabolite produced by Aspergillus terreus which is used as cholesterol-lowering drugs called HMG-CoA reductase inhibitors. In the present study to increase the yield of lovastatin, production media components were optimized. The impact of media components which includes carbon, nitrogen source, organic and inorganic salts were studied with response surface methodology. A “Plackett-Burman Design” was employed to evaluate the effects of different components in the media. The concentration of Casein, Citric acid and Sodium acetate significantly influence the Lovastatin production. The Pareto chart was employed to determine the optimal regions of these three significant factors. These three factors were optimized using central composite design (CCD) of “response surface methodology.” The optimized fermentation medium composition was as follows (g/L): Casein 25.0, Citric acid 12.0, Sodium acetate 18.0, Dextrose 40.0, Cotton seed meal 5.50, Soya flour 20.5, Magnesium sulphate (MgSO4.7H2O) 1.0, Calcium carbonate 7.5, Glycerol 5.0, PEG-400 3.85, Soya oil 5.0. The validated model can precisely predict the Lovastatin production.
Authors and Affiliations
Umesh Luthra
Keywords
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