Media Optimization For Lovastatin Production by Statistical Approach Using Aspergillus terreus by Submerged Fermentation
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 2
Abstract
Lovastatin is a secondary metabolite produced by Aspergillus terreus which is used as cholesterol-lowering drugs called HMG-CoA reductase inhibitors. In the present study to increase the yield of lovastatin, production media components were optimized. The impact of media components which includes carbon, nitrogen source, organic and inorganic salts were studied with response surface methodology. A “Plackett-Burman Design” was employed to evaluate the effects of different components in the media. The concentration of Casein, Citric acid and Sodium acetate significantly influence the Lovastatin production. The Pareto chart was employed to determine the optimal regions of these three significant factors. These three factors were optimized using central composite design (CCD) of “response surface methodology.” The optimized fermentation medium composition was as follows (g/L): Casein 25.0, Citric acid 12.0, Sodium acetate 18.0, Dextrose 40.0, Cotton seed meal 5.50, Soya flour 20.5, Magnesium sulphate (MgSO4.7H2O) 1.0, Calcium carbonate 7.5, Glycerol 5.0, PEG-400 3.85, Soya oil 5.0. The validated model can precisely predict the Lovastatin production.
Authors and Affiliations
Umesh Luthra
Keywords
Related Articles
- EP ID EP210908
- DOI -
- Views 97
- Downloads 0
MRI Characterisation of Focal Liver Lesions- A Tertiary Care Centre Study
Back ground: The role of MRI is more rewarding as it is known that MRI details of soft tissue is superior than CT or any other imaging modalities and this quality is utilized in the evaluation of focal liver lesions, apa...
Knowledge Regarding Cervical Cancer among Women above 35 Years
A non experimental descriptive design with cross sectional survey approach was undertaken to assess the knowledge regarding cervical cancer among women above 35 years in Chinna seeragapadi village, Salem. It was conclued...
Prevalence of Primary Open-Angle Glaucoma in Diabetics Attending Postgraduate Department of Ophthalmology GMC Srinagar
Background: In 1971 Becker stated “diabetes mellitus occurs more often in patients with primary open angle glaucoma than in non-glaucomatous populations. Armstrong et al reported a prevalence of POAG of 4.1% in the diabe...
Preoperative Predictors for Conversion in Laparoscopic Cholecystectomy
Introduction: Laparoscopic cholecystectomy has rejuvenated general surgery and in very short time has become the gold standard operation for benign gallbladder disease, but the procedure is technically more demanding tha...
Alteration of Gene Expression Due to Arsenic Induced Oxidative Stress Leading to Ovarian Cancer
Epidemiological studies and preventive measures for cancer have engaged most of the scientists and doctors for several decades and the research has come forth a long way now. Several heavy metals like arsenic have been l...