Methanolic Leaf Extract of Diospyros chloroxylon Modulates Hepatic Redox Profile and Cell Proliferation in Dimethylamine-treated Rats
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2017, Vol 21, Issue 12
Abstract
Dimethylamine (DMA) is a toxicant commonly present in foods, air and water. Diospyros species is a group of plants being reported to contain several bioactive agents. The study was aimed at investigating the biological activities of Diospyros chloroxylon leaf extract (DCLE) on DMA-induced toxicity in liver of Wistar rats. The DMA significantly (p < 0.05) reduced serum Superoxide dismutase (SOD) and catalase activities by 43.9% and 60.6%, while malondialdehyde (MDA) was found to be significantly (p < 0.05) elevated by 150% compared with controls. Hepatic SOD and catalase activities, and reduced glutathione (GSH) level were significantly (p < 0.05) reduced by 49.9%, 32% and 26.3%, respectively, while MDA level was increased by 94.5% in DMA group against controls. The DCLE significantly (p < 0.05) increased SOD and catalase activities, and lowered MDA level in serum and liver against DMA treatment. DNA fragmentation was observed to be significantly elevated by 42.5% in DMA-treated rats compared with controls. Pretreatment with DCLE significantly (p < 0.05) lowered DNA fragmentation in the [DCLE + DMA] group. Histological data reveal that DMA caused periportal cell infiltration in hepatocytes, whereas the control, DCLE and [DCLE + DMA] groups showed no visible lesion. Immunohistochemical (IHC) staining of CD34 showed a strong expression in DMA group, while DCLE + DMA] showed a moderate expression. This study thus suggests that methanolic leaf extract of Diospyros chloroxylon ameliorates oxidative stress, DNA damage and cell proliferation in liver of rats treated with Dimethylamine.
Authors and Affiliations
G. E. Adeleke, O. T. Adedosu, O. K. Afolabi, O. O. Arinde, T. M. Oyedokun
Keywords
Related Articles
- EP ID EP311880
- DOI 10.9734/BJMMR/2017/33242
- Views 56
- Downloads 0
Dynamic Anatomy of Aortic Root and Its Potential Role in TAVR Prostheses Further Development and Modification
Aortic stenosis (AS) is the most common valvular disorder. Surgical aortic valve replacement is the definitive therapy for patients with severe AS. In the last years transcatheter aortic valve replacement (TAVR) has been...
The Hsp90 Chaperone Machinery: An Important Hub in Protein Interaction Networks
Heat shock protein 90 (Hsp90) represents one of the most conserved proteins in living organisms and is present in all kingdoms of life except for Archaea. HSP90 proteins define a widespread family of molecular chaperones...
Serum Vitamin D Concentration in Asthmatic Children and Its Association with Recovery Time from an Asthma Exacerbation
Introduction: The relationship of vitamin D and asthma is known but the association between vitamin D and time required to recover from acute asthma exacerbation has not been studied. Aims: To find an association betwee...
Gingival Depigmentation Using Lasers: A Literature Review
Background: Gingival hyperpigmentation usually occurs due to the abnormal accumulation of melanin in the gingival tissue and confers a dark appearance to the gingiva. Gingival hyperpigmentation often compromises smile es...
Supernumerary Molars: A Rare Finding
Panoramic radiographs of a female patients aged twenty one years without any associated syndromic or systemic medical history, revealed the presence of the two supernumerary molars, right upper and left lower fourth mola...