Method Development a nd Validation o f Simultaneous Estimation o f Pseudoephedrine Ambroxol Deslo ratadine in Tablet Dosage Form a nd Degradation Studies By Rp - Hplc Method
Journal Title: International Journal of Scientific Research and Management - Year 2017, Vol 5, Issue 7
Abstract
Method Development a nd Validation o f Simultaneous Estimation o f Pseudoephedrine Ambroxol Deslo ratadine in Tablet Dosage Form a nd Degradation Studies By Rp - Hplc Method A simple, Accurate, precise method was developed for the simultaneous estimation of the Pseudoephedrine, Ambroxol and Desloratadine in Tablet dosage form. Chromatogram was run through ODS 250mm x 4.6 mm, 5 . Mobile phase containing Buffer, Acetonitrie and Methanol in the ratio of 50:20A:30M was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.01N KH 2 PO 4 buffer at pH 3.2. Temperature was maintained at 30°C. Optimized wavelength for Pseudoephedrine and Ambroxol was 225nm. Retention time of Pseudoephedrine, Ambroxol and Desloratadine were found to be 2.417min, 4.518min and 7.464min respectively. %RSD of the Pseudoephedrine, Ambroxol and Desloratadine were and found to be 1.03 0.64 and 1.15respectively. %Recover was Obtained as 99.92%, 100.21% and 99.92% for Pseudoephedrine, Ambroxol a nd Desloratadine respectively. LOD, LOQ values are obtained from regression equations of Pseudoephedrine(0.01ppm, 0.02ppm), Ambroxol(0.15ppm, 1.44ppm) and Desloratadine(0.06ppm, 0.17ppm). Regression equation of Pseudoephedrine is y = 16947x + 319.2 Ambroxo l is y = 19401x + 3236. and Desloratidine y = 38993x +2051.Method Development a nd Validation o f Simultaneous Estimation o f Pseudoephedrine Ambroxol Deslo ratadine in Tablet Dosage Form a nd Degradation Studies By Rp - Hplc Method A simple, Accurate, precise method was developed for the simultaneous estimation of the Pseudoephedrine, Ambroxol and Desloratadine in Tablet dosage form. Chromatogram was run through ODS 250mm x 4.6 mm, 5 . Mobile phase containing Buffer, Acetonitrie and Methanol in the ratio of 50:20A:30M was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.01N KH 2 PO 4 buffer at pH 3.2. Temperature was maintained at 30°C. Optimized wavelength for Pseudoephedrine and Ambroxol was 225nm. Retention time of Pseudoephedrine, Ambroxol and Desloratadine were found to be 2.417min, 4.518min and 7.464min respectively. %RSD of the Pseudoephedrine, Ambroxol and Desloratadine were and found to be 1.03 0.64 and 1.15respectively. %Recover was Obtained as 99.92%, 100.21% and 99.92% for Pseudoephedrine, Ambroxol a nd Desloratadine respectively. LOD, LOQ values are obtained from regression equations of Pseudoephedrine(0.01ppm, 0.02ppm), Ambroxol(0.15ppm, 1.44ppm) and Method Development a nd Validation o f Simultaneous Estimation o f Pseudoephedrine Ambroxol Deslo ratadine in Tablet Dosage Form a nd Degradation Studies By Rp - Hplc Method A simple, Accurate, precise method was developed for the simultaneous estimation of the Pseudoephedrine, Ambroxol and Desloratadine in Tablet dosage form. Chromatogram was run through ODS 250mm x 4.6 mm, 5 . Mobile phase containing Buffer, Acetonitrie and Methanol in the ratio of 50:20A:30M was pumped through column at a flow rate of 1ml/min. Buffer used in this method was 0.01N KH 2 PO 4 buffer at pH 3.2. Temperature was maintained at 30°C. Optimized wavelength for Pseudoephedrine and Ambroxol was 225nm. Retention time of Pseudoephedrine, Ambroxol and Desloratadine were found to be 2.417min, 4.518min and 7.464min respectively. %RSD of the Pseudoephedrine, Ambroxol and Desloratadine were and found to be 1.03 0.64 and 1.15respectively. %Recover was Obtained as 99.92%, 100.21% and 99.92% for Pseudoephedrine, Ambroxol a nd Desloratadine respectively. LOD, LOQ values are obtained from regression equations of Pseudoephedrine(0.01ppm, 0.02ppm), Ambroxol(0.15ppm, 1.44ppm) and Desloratadine(0.06ppm, 0.17ppm). Regression equation of Pseudoephedrine is y = 16947x + 319.2 Ambroxo l is y = 19401x + 3236. and Desloratidine y = 38993x +2051.Desloratadine(0.06ppm, 0.17ppm). Regression equation of Pseudoephedrine is y = 16947x + 319.2 Ambroxo l is y = 19401x + 3236. and Desloratidine y = 38993x +2051.
Authors and Affiliations
Mohammad Mubina
Keywords
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