Method Development and Validation for Simultaneous Estimation of telithromycin and Ketoprofen by RP-HPLC
Journal Title: World Journal of Pharmaceutical Sciences - Year 2018, Vol 6, Issue 5
Abstract
The present work describes a simple, rapid, and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of telithromycin (TLM) and ketoprofen (KP). C18 column (chromosil ODS, 4 𝜇m, 200× 4.0mm) and a mobile phase containing phosphate buffer (0.05 M) along with 1-octane sulphonic acid sodium salt monohydrate (0.005 M) adjusted to pH 3.2: acetonitrile (45 : 55 v/v) mixture was used for the separation and quantification. The flow rate was0.8 mL/min and the eluents were detected by UV detector at 225 nm. The retention times were found to be 3.48 and 5.42 mins, respectively.The developed method was validated according to ICH guidelines Q2 (R1) and found to be linear within the range of 75–175 𝜇g/mL for both drugs. The developed method was applied successfully for assay of telithromycin and ketoprofen in their combined in-house developed dosage forms and in vitro dissolution studies.
Authors and Affiliations
Kishore M
Keywords
Related Articles
- EP ID EP505772
- DOI -
- Views 65
- Downloads 0
Removal of Mordant Green 17 and Acid Fast Red from Water using Nanokaolin
Increasing concentrations of dyes including mordant green17 and acid fast RN in surface and groundwater are a major environmental concern in recent years. In this research, the potential of nanokaolin for efficient remov...
Personality factors and Coping Skills among Coronary Heart Disease Patients
Coronary heart disease is a leading cause of death and a cause of disability worldwide. Personality factors and coping skills are important determinants of morbidity and mortality associated with Coronary artery disease....
A study on knee joint effusion with osteoarthritis by musculoskeletal ultrasonography- before and after management
AIM: The aim of study is to investigate the changes of knee joint effusion before and after osteoarthritis of knee, using musculoskeletal Ultrasonograpy. DESIGN: Prospective, follow-up study. SETTING: Department of Physi...
Synthesis, characterization, antimicrobial studies and computational studies of a new Shciff‘s base ligand derived from 3-aminocoumarin and its metal complexes
The novel Schiff’s base ligand (OCYMB) 3 synthesized by the condensation of o-phthalaldehyde and 3- aminocoumarin. The nature bonding and geometry of the transition metal complexes as well as ligand have been deduced fro...
Stability evaluation of Phyllanthus Urinaria extract loaded palm kernel oil esters-based nanoemulsions
Storage stability evaluation of Phyllanthus urinaria extract loaded palm kernel oil esters-based nanoemulsion creams was carried at various environmental conditions for duration of three months. The formulations were eva...