Method development and validation for simultaneous estimation of telmisartan and amlodipine by RP-HPLC
Journal Title: World Journal of Pharmaceutical Sciences - Year 2017, Vol 5, Issue 4
Abstract
A novel, precise, accurate, rapid and effective isocratic RP-HPLC method was developed, optimized and validated for the estimation of Telimsartan (TEL) and Amlodipine (AML) in pharmaceutical dosage forms (tablet). The drugs were estimated using Symmetry C18 (250 x 4.6 mm, 5 µm) column. A mobile phase composed of phosphate buffer of pH 6 and acetonitrile in the ratio of 40:60, v/v), at a flow rate of 0.8 ml/min was used for the separation. Detection was carried out at 243 nm. The linearity range obtained was 16-48 µg/ml for TEL and 2-6 µg/ml for AML with retention times (Rt) of 3.209 min and 5.351 min for TEL and AML respectively. The correlation coefficient values were found to be 0.999. Precession studies showed % RSD values less than 2 % for both the drugs in all the selected concentrations. The percentage recoveries of TEL and AML were in the range of 98.01-101.62% and 99.30-101.40% respectively. The assay results of TEL and AML were 99.60% and 99.75% respectively. The method was validated as per the International Conference on Harmonization (ICH) guidelines. The developed validated method was successfully used for the quantitative analysis of commercially available dosage form.
Authors and Affiliations
Vatchavai Bhaskara Raju
Keywords
Related Articles
- EP ID EP328312
- DOI -
- Views 97
- Downloads 0
Alzheimer’s – An updated review on disease and its treatment
Alzheimer’s disease (AD) is the most common cause of cognitive impairment in older patients and is expected to increase greatly in prevalence in the next future. An excess of senile plaques (h-amyloid protein) and neurof...
Assessment of knowledge, awareness and reporting of pharmacovigilance among the healthcare providers in a tertiary care center
There is a considerable increase in awareness about the issues related to drug safety among health care providers, healthcare institutions and the public. Our aim of this present study was to assess the knowledge; awaren...
Pharmacological Activity of Nigella Sativa: A Review
Nigella sativa (NS) are dark, thin, and crescent-shaped, seeded shrub belonging to the family Ranunculaceae, commonly growing on Mediterranean coasts in Saudi Arabia, northern Africa and Asia. It is a widely used medicin...
Preparation and evaluation of anti-hyperlipidaemic in topical preparation and characterization using vesicular drug delivery system
Simvastatin is an HMG CoA reductase inhibitor used for the treatment of dyslipidemia, however the drug has two major problems; short biological half‐life and extensive first pass effect. The aim of this work was to devel...
Effect of resveratrol on cardio vascular system
Resveratrol (RESV) is a polyphenolic compound found in various plants, including grapes, berries and peanuts, and its processed foods as red wine. RESV possesses a variety of bioactivities, including antioxidant, anti-in...