METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF SAROGLITAZAR IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC
Journal Title: World Journal of Pharmaceutical Research - Year 2017, Vol 6, Issue 9
Abstract
A simple, sensitive and rapid reverse phase high performance liquid chromatographic method was developed for the estimation of Saroglitazar in tablet dosage form. A C18 Inertsil ODS column (250 × 4.6 mm, 5μm particle size) was used as a stationary phase with a mobile phase containing a mixture of phosphate buffer and acetonitrile in the ratio of 40:60, v/v. The flow rate was 1.0 mL/min. The effluent was monitored at 294 nm and eluted at 2.162 min. Calibration curve was plotted with a range from 20-100 μg/ml for Saroglitazar and the correlation was found to be 0.999. The accuracy range was found between 99.95 -101.07%. The % RSD values for both intraday and interday precision were less than 2.0. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.405μg/ml and 4.260μg/ml respectively. The assay was validated for the parameters like system suitability, precision, accuracy, and robustness parameters. The proposed method can be useful for the routine determination of Saroglitazar in pharmaceutical dosage form.
Authors and Affiliations
Nita Yadav
Keywords
Related Articles
- EP ID EP673661
- DOI -
- Views 165
- Downloads 0
SHUKRA BEEJA KSHEENATA IN REFERENCE TO OLIGOSPERMIA- A LITERARY REVIEW
To continue the world reproduction is necessary for all animals. For reproduction in human being the availability of sperm and ova is necessary. Although there is requirement of one sperm to fertilize the ovum but the no...
SOLID STATE SINTERING: A NOVEL TECHNIQUE FOR CONTROLLED RELEASE
Sintering is defined as the bonding of adjacent particle surfaces in a mass of powder or in a compact by application of heat. This concept in pharmaceutical science is relatively recent, but research interests relating t...
AN AYURVEDA REVIEW ON CAUSE, MANAGEMENT AND PREVENTION OF YUVANA PIDIKA (ACNE VULGARIS)
The concept of beauty has prevailed since mankind earliest ages but in today’s world beauty is one of the most valued and desirable attributes. It is that quality or combination of qualities which afford keen pleasure to...
EXTRACTION OF NATURAL COLORANTS FROM TWO VARIETIES OF SAFFRON (CROCUS SATIVUS) AND THEIR DYEING EVALUATION ON GOAT LEATHER
The colorant was extracted from Iranian and Italian crocus sativus stamen by maceration, mixing both the samples separately in 300 mL methanol (solvent). Mixture was kept in dark for 36 hours, filtered and then each filt...
PREVALENCE OF IRON DEFICIENCY IN CHILDREAN WITH FEBRILE SEIZURE
Objective: Febrile seizures are one of the most common neurologic problems during infancy and childhood periods, occurring in 3-4% of the children with a recurrence rate of 30 to 40%. They occur rarely before 6 months an...