METHOD DEVELOPMENT AND VALIDATION OF FAST DISSOLVING TABLET OF RAMIPRIL BY HPLC METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 3
Abstract
Objective: The Objective of present work is method development and validation of HPLC method for the quantitation of Ramipril in Fast dissolving tablet (FDT).Methods: A stable, linear, rapid, accurate and selective HPLC method has been developed for the quantification of Ramipril in FDT using buffer and acetonitrile: methanol (60:40 v/v) ratio in combination as mobile phase and at the flow rate of 1 ml/minute at λmax 210 nm. Chromatographic separation was performed on Shimadzu SPD-20A, SD-M10 AVP-Shimadzu, an ODS C-18 Kromacil (250 mm × 4.60 mm) column used as stationary phase. The quantitation of Ramipril done by HPLC, parameters studied were retention time, linearity, accuracy, precision, detection limit, quantitation limit and stability.Results: Linear regression analysis data show a good linear relationship between response and concentration in the range of 5-30 µg/ml; detection carried out at λmax210 nm; the linear regression equation for Ramipril was Y=10327x+72877; R²=0.998. The retention time of the Ramipril was 2.910 min. Percent recoveries obtained for Ramipril was 99.58-100.15%. LOD and LOQ value was 0.802µg/ml and 1.4µg/ml for Ramipril respectively.Conclusion: The result suggested that proposed method gives good peak resolution of Ramipril within short analysis time (<10 min) and high percentages of the recovery shown that method is free from interference of excipient present in the formulation. The % RSD of each parameter lies below the limit of 2%, proven the suitability. The statistical analysis proved that the proposed method is precise, accurate, selective and rapid for the HPLC estimation of Ramipril.Keywords: Fast dissolving tablet, Ramipril, Accuracy, HPLC, Linearity
Authors and Affiliations
Vaibhav Rajoriya, Amrita Soni, Varsha Kashaw
Keywords
Related Articles
- EP ID EP577789
- DOI -
- Views 48
- Downloads 0
UTILIZATION OF ION-PAIR COMPLEX FORMATION FOR THE SPECTROPHOTOMETRIC DETERMINATION OF SOME ANTIDEPRESSANT DRUGS IN PHARMACEUTICAL FORMULATIONS
Objective: A new, simple and sensitive spectrophotometric method has been developed for the determination of some antidepressant drugs, namely desvenlafaxine (DSV), dapoxetine (DAP) and citalopram (CIT) in pharmaceutical...
SYNTHESIS AND CHARACTERIZATION IN VITRO ANTIMICROBIAL AND CYTOTOXICITY TESTING OF OXALIC ACID-DERIVED CADMIUM CHELATING AGENTS
Objective: The aim of this study is to investigate the growth, structure, spectral, solubility and biological activity of sodium cadmium oxalate dehydrate (NaCdOx) and cadmium oxalate trihydrate (CdOx) crystals prepared...
RELATIONSHIP BETWEEN PERIODONTAL DISEASE AND ABO BLOOD GROUP PHENOTYPES-A CROSS SECTIONAL RETROSPECTIVE STUDY
Objective: Numerous epidemiological studies have been carried out to assess the association of ABO blood typing and systemic diseases. However, the research is still at the infancy stage with regards to the dental field....
ANTI-ULCER AND ANTIOXIDANT ACTIVITY OF NELUMBO NUCIFERA GAERTN STALKS IN RATS
Objective: Nelumbo nucifera Gaertn. (Nymphaeceae) is a well known aquatic herb which has been extensively used traditionally for the treatment of different diseases. Therefore present study was designed to evaluate the a...
CURCUMIN-ZN-ARTEMETHER COMBINATION THERAPY FOR PLASMODIUM BERGHEI INFECTED MICE
Objective: Studies have shown that a new combination therapy with artemisinin derivatives and curcumin is unique, with potential advantages over known Artemisinin Combination Therapy (ACT). The problems of poor solubilit...